Bonner, Tom I. and Brenner, Don J. and Neufeld, Berney R. and Britten, Roy J. (1973) Reduction in the rate of DNA reassociation by sequence divergence. Journal of Molecular Biology, 81 (2). pp. 123-135. ISSN 0022-2836. doi:10.1016/0022-2836(73)90184-8. https://resolver.caltech.edu/CaltechAUTHORS:20160414-122552695
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Abstract
An estimate is made of the effect of imperfectly complementary sequences on the rate of reassociation of DNA. Rate measurements are reported for the reassociation of deaminated DNA and for the pairing of DNAs from related bacteria. A method is presented for separating the effect of the incubation temperature on the rate from the effect of sequence divergence. After correction to the optimum incubation temperature, the rate of DNA reassociation appears to be reduced by a factor of two for each 10 deg. C reduction in melting temperature due to sequence divergence. For most typical cases this effect is modest. However it can be quite important for measurements of the relation between the DNAs of different species.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1973 Elsevier. (Received 15 March 1973) We are indebted to G. R. Fanning, A. G. Steigerwalt, J. Miklos and J. Smith for technical assistance in parts of this work. This work was supported in part by U.S. Public Health Service grant no. HD05753. One of us (B. R. N.) is a U.S. Public Health Service Fellow and another author (T. I. B.) is a Carnegie Institution of Washington Fellow. | |||||||||
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| Issue or Number: | 2 | |||||||||
| DOI: | 10.1016/0022-2836(73)90184-8 | |||||||||
| Record Number: | CaltechAUTHORS:20160414-122552695 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160414-122552695 | |||||||||
| Official Citation: | Tom I. Bonner, Don J. Brenner, Berney R. Neufeld, Roy J. Britten, Reduction in the rate of DNA reassociation by sequence divergence, Journal of Molecular Biology, Volume 81, Issue 2, 1973, Pages 123-135, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(73)90184-8. (http://www.sciencedirect.com/science/article/pii/0022283673901848) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 66171 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | George Porter | |||||||||
| Deposited On: | 15 Apr 2016 20:41 | |||||||||
| Last Modified: | 10 Nov 2021 23:54 |
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