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Evolutionary change in the repetition frequency of sea urchin DNA sequences

Moore, Gordon P. and Scheller, Richard H. and Davidson, Eric H. and Britten, Roy J. (1978) Evolutionary change in the repetition frequency of sea urchin DNA sequences. Cell, 15 (2). pp. 649-660. ISSN 0092-8674. https://resolver.caltech.edu/CaltechAUTHORS:20160414-125227868

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Abstract

The frequency of occurrence of particular repetitive sequence families has been estimated in the DNA of the three sea urchin species Strongylocentrotus purpuratus, Strongylocentrotus franciscanus and Lytechinus pictus using individual cloned S. purpuratus repetitive sequence elements. Cloned repetitive sequence elements as described by Scheller et al. (1977a) were prepared by reassociation of S. purpuratus DNA fragments to repetitive Cot, digestion with single-strand-specific nuclease S1 and ligation of synthetic restriction sites to their ends. The sequences were cloned by insertion at the Eco RI site of plasmid RSF2124, labeled, strand-separated and reassociated with 800–900 nucleotide long unlabeled DNA. Both kinetic (genomic DNA excess) and saturation (cloned DNA excess) estimates of frequencies were made. For nine cloned fragments, the ratio of the repetition frequency in S. purpuratus DNA to that in S. franciscanus DNA ranges from about 20 to about 1. In the four cases examined, only a few copies were detected in the DNA of L. pictus. Estimates have also been made of frequency changes in many repetitive families by measuring the reassociation of labeled repetitive DNA fractions of each species with total DNA from other species. In each reciprocal comparison, the labeled repetitive sequences reassociate more slowly with DNA of other species than with DNA of the species from which they were prepared. Thus it appears that the dominant repetitive sequence families in the DNA of each species are present at lower frequencies in the DNA of closely related species. Measurements of thermal stability have been made of S. purpuratus cloned repetitive sequences reassociated with S. franciscanus DNA or S. purpuratus DNA. Most families have changed both in frequency and sequence, although some have changed little in sequence but show great changes in frequency.


Item Type:Article
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http://dx.doi.org/10.1016/0092-8674(78)90033-8DOIArticle
http://www.sciencedirect.com/science/article/pii/0092867478900338PublisherArticle
Additional Information:© 1978 MIT. Received May 1, 1978; revised June 24, 1978. The knowledge before publication of the measurements of P. Dunsmuir, P. Bingham and M. Meselson with Drosophila species was helpful in recognizing the generality and significance of frequency change. Unpublished measurements made by Dr. Robert C. Angerer of the conservation of repetitive DNA sequences in these sea urchins were also valuable. We acknowledge the excellent technical assistance of Linda Vock and Richard Rohan. This work has been supported by a grant from the National Institute of General Medical Sciences. G. P. M. is the recipient of a fellowship from the American Cancer Society. R. H. S. is a predoctoral fellow on an NIH training grant. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
National Institute of General Medical SciencesUNSPECIFIED
American Cancer SocietyUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160414-125227868
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160414-125227868
Official Citation:Gordon P. Moore, Richard H. Scheller, Eric H. Davidson, Roy J. Britten, Evolutionary change in the repetition frequency of sea urchin DNA sequences, Cell, Volume 15, Issue 2, 1978, Pages 649-660, ISSN 0092-8674, http://dx.doi.org/10.1016/0092-8674(78)90033-8. (http://www.sciencedirect.com/science/article/pii/0092867478900338)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66176
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:15 Apr 2016 23:01
Last Modified:03 Oct 2019 09:54

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