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Specific representation of cloned repetitive DNA sequences in sea urchin RNAs

Scheller, Richard H. and Costantini, Franklin D. and Kozlowski, Michael R. and Britten, Roy J. and Davidson, Eric H. (1978) Specific representation of cloned repetitive DNA sequences in sea urchin RNAs. Cell, 15 (1). pp. 189-203. ISSN 0092-8674. doi:10.1016/0092-8674(78)90094-6.

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Nine cloned repetitive sequences were labeled, strand-separated and individually hybridized with RNA extracted from the nuclei of gastrula stage sea urchin embryos and of adult sea urchin intestine cells. The concentration of transcripts complementary to each cloned sequence was measured by RNA excess hybridization kinetics and by a DNA excess titration method. Transcripts of certain of the repeat families are present at over 100 times the concentration of transcripts of other families in each RNA. The set of repetitive sequence families highly represented in intestine nuclear RNA is different from that highly represented in gastrula nuclear RNA. Together with the results obtained with mature oocyte RNA and presented in the accompanying paper by Costantini et al. (1978), these findings show that quantitative patterns of repetitive sequence representation in RNA are specific to each cell type. Both strands of all of the nine cloned repeats are represented at some level in all the RNAs studied. Usually, though not always, the concentrations of transcripts complementary to the two strands of each repeat do not differ by more than a factor of two. The cloned tracers do not react with polysomal messenger RNA, and the nuclear RNA molecules with which they hybridize are many times larger than the repetitive sequences themselves.

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Additional Information:© 1978 MIT. Received April 13, 1978; revised June 8, 1978. We wish to acknowledge the expert assistance of Mr. Clifford Beall. This research was supported by a grant from the NSF. R.H.S. and F.D.C. were supported by NIH training grants to the Division of Chemistry and to the Division of Biology. The P2 facilities used in this work were equipped with the aid of funds from a Biomedical Research Support Grant. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S .C. Section 1734 solely to indicate this fact.
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NIH Predoctoral FellowshipUNSPECIFIED
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Record Number:CaltechAUTHORS:20160414-134909791
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Official Citation:Richard H. Scheller, Franklin D. Costantini, Michael R. Kozlowski, Roy J. Britten, Eric H. Davidson, Specific representation of cloned repetitive DNA sequences in sea urchin RNAs, Cell, Volume 15, Issue 1, 1978, Pages 189-203, ISSN 0092-8674, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66177
Deposited By: George Porter
Deposited On:15 Apr 2016 23:07
Last Modified:10 Nov 2021 23:54

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