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Sea urchin embryo mRNA sequences expressed in the nuclear RNA of adult tissues

Wold, Barbara J. and Klein, William H. and Hough-Evans, Barbara R. and Britten, Roy J. and Davidson, Eric H. (1978) Sea urchin embryo mRNA sequences expressed in the nuclear RNA of adult tissues. Cell, 14 (4). pp. 941-950. ISSN 0092-8674. https://resolver.caltech.edu/CaltechAUTHORS:20160414-140621610

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Abstract

The representation of message sequences in nuclear RNA was studied in sea urchin tissues which utilize these messages in their polysomes and in tissues which do not. A ^3H-labeled single-copy DNA tracer highly enriched for sequences complementary to blastula embryo mRNA was prepared. This tracer (mDNA) reacted to 78% with excess blastula mRNA, compared with 2.1% for the starting single-copy DNA. As expected from previous data, the mDNA reacted to only 12% with cytoplasmic RNA of adult intestine, since most embryo mRNA sequences are not detectable in this tissue. The mDNA reacted to the same extent, however, with nuclear RNA from either adult intestine, adult coelomocytes or gastrula embryos as with polysomal mRNA from blastula embryos. Thus virtually all of the blastula mRNA sequences are present in adult tissue nuclear RNAs, although most of these mRNA sequences are absent from cytoplasmic or polysomal RNA in the adult cells. The blastula mRNA sequences are present in heterogeneous nuclear RNAs at the same concentrations as are nuclear transcripts of most single-copy DNA sequences. Calculations based on the steady state concentration of structural gene transcripts in the embryo nuclei suggest that a majority of these molecules do not serve as message precursors.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0092-8674(78)90348-3DOIArticle
http://www.sciencedirect.com/science/article/pii/0092867478903483PublisherArticle
ORCID:
AuthorORCID
Wold, Barbara J.0000-0003-3235-8130
Additional Information:© 1978 MIT. Received May 1, 1978. This research was supported by an NIH grant. B.J.W. was supported by an NSF predoctoral fellowship and a Danforth Foundation fellowship. W.H.K. was supported by a Lievre fellowship from the American Cancer Society, California Division. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked “advertisement” in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
NSF Predoctoral FellowshipUNSPECIFIED
Danforth FoundationUNSPECIFIED
American Cancer Society, California DivisionLievre Fellowship
Issue or Number:4
Record Number:CaltechAUTHORS:20160414-140621610
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160414-140621610
Official Citation:Barbara J. Wold, William H. Klein, Barbara R. Hough-Evans, Roy J. Britten, Eric H. Davidson, Sea urchin embryo mRNA sequences expressed in the nuclear RNA of adult tissues, Cell, Volume 14, Issue 4, 1978, Pages 941-950, ISSN 0092-8674, http://dx.doi.org/10.1016/0092-8674(78)90348-3. (http://www.sciencedirect.com/science/article/pii/0092867478903483)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66179
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:15 Apr 2016 22:59
Last Modified:09 Mar 2020 13:19

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