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Appearance and persistence of maternal RNA sequences in sea urchin development

Hough-Evans, Barbara R. and Wold, Barbara J. and Ernst, Susan G. and Britten, Roy J. and Davidson, Eric H. (1977) Appearance and persistence of maternal RNA sequences in sea urchin development. Developmental Biology, 60 (1). pp. 258-277. ISSN 0012-1606. http://resolver.caltech.edu/CaltechAUTHORS:20160414-143901784

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Abstract

This paper deals with the relationship between the single copy transcripts represented in mature oocytes of the sea urchin and the RNA sequences present in immature oocytes and embryos. We term the oocyte transcripts from single copy DNA the maternal single copy sequence set. A single copy [^3H]DNA fraction ([^3H]oDNA) enriched for sequences complementary to the maternal single copy sequence set was prepared and reacted with the different RNA preparations. The complexity of the mature oocyte RNA is estimated to be 37 × 10^6 nucleotides. At kinetic termination, [3H]oDNA reacted with the polysomal mRNA of 16-cell embryos to 73% of the reaction with mature oocyte RNA, indicating that 27 × 10^6 nucleotides of the maternal sequence set are present. With blastula mRNA the reaction equals about 56%, a complexity of 21 × 10^6 nucleotides; with gastrula mRNA, 53%, a complexity of 19 × 10^6 nucleotides. The relative amount of hybridization of [^3H]oDNA was 100% with cytoplasmic RNA of the 16-cell stage and became progressively less with the cytoplasmic RNAs of later stages. The total RNA of immature oocytes was found to include about 26 × 10^6 nucleotides of the maternal sequence set. Results of these experiments are discussed, and an interpretation of the pattern of utilization of structural genes during oocyte and embryo development is suggested.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0012-1606(77)90123-3DOIArticle
http://www.sciencedirect.com/science/article/pii/0012160677901233PublisherArticle
Additional Information:© 1977 Elsevier. Received April 21, 1977; accepted in revised form June 9, 1977. We are pleased to acknowledge the competent and intelligent technical assistance of Michael R. Kozlowski. This research was supported by grants from the NIH (Child Health and Human Development and Research Resources) and the NSF. B.J.W. has been supported by an NSF Fellowship and by the McCallum and Danforth Funds. S.G.E. was supported by the National Foundation-March of Dimes and by an NIH Fellowship.
Funders:
Funding AgencyGrant Number
National Institute of Child Health and Human Development (NICHD)UNSPECIFIED
NSF Graduate Research FellowshipUNSPECIFIED
McCallum FellowshipUNSPECIFIED
Danforth FoundationUNSPECIFIED
National Foundation - March of DimesUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Record Number:CaltechAUTHORS:20160414-143901784
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160414-143901784
Official Citation:Barbara R. Hough-Evans, Barbara J. Wold, Susan G. Ernst, Roy J. Britten, Eric H. Davidson, Appearance and persistence of maternal RNA sequences in sea urchin development, Developmental Biology, Volume 60, Issue 1, 1977, Pages 258-277, ISSN 0012-1606, http://dx.doi.org/10.1016/0012-1606(77)90123-3. (http://www.sciencedirect.com/science/article/pii/0012160677901233)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66181
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:15 Apr 2016 20:28
Last Modified:13 Feb 2019 18:06

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