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Significance of rare mRNA sequences in liver

Galau, Glenn A. and Klein, William H. and Britten, Roy J. and Davidson, Eric H. (1977) Significance of rare mRNA sequences in liver. Archives of Biochemistry and Biophysics, 179 (2). pp. 584-599. ISSN 00039861. http://resolver.caltech.edu/CaltechAUTHORS:20160418-085641040

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Abstract

From mRNA-DNA hybridization studies it is known that eukaryotic mRNAs occur in several abundance classes. One such study with mammalian liver mRNA indicates that in this tissue the most complex abundance class consists of messenger RNAs present in 5–40 copies per cell. The other abundance classes are less complex and consist of mRNA sequences present an average of 250 and 7200 times per cell. In this paper we summarize approximate calculations of the number of mRNA molecules needed to sustain the steady-state quantities of 40 rodent liver proteins. Data were obtained from the literature regarding subunit molecular weights, degradation rate constants, and the concentration of each of these proteins in liver. The sample of proteins was chosen simply on the basis of the availability of the relevant data in the literature. For 13 of these proteins more direct estimates of mRNA frequency per cell could also be derived from measurements of the fraction of total protein synthesis accounted for by the protein in question. In all cases these estimates agreed within a factor of 2 to 3 with the values calculated from protein concentration and turnover rates. Several proteins known to be expressed in a histospecific way in liver were found to require steady-state mRNA concentrations in the range of only 1–30 copies per cell. This suggests that at least some of the mRNAs in the lowest abundance class are present as the result of the specific regulation of structural gene transcription.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0003-9861(77)90147-3DOIArticle
http://www.sciencedirect.com/science/article/pii/0003986177901473PublisherArticle
Additional Information:© 1977 Academic Press. Received August 19, 1976. This work was supported by grants from the National Institutes of Health (HD-05753 and GM-20927) and by a grant from the National Science Foundation (BMS7507359). G.A.G. was supported by U.S. Public Health Training Grant (GM-000861 and by The National Foundation March of Dimes Grant (1-404). W.H.K. was supported by Damon Runyon-Walter Winchell Cancer Fund Grant (DRG-19-F) and by American Cancer Society, California Division, Lievre Fellowship (J-340).
Funders:
Funding AgencyGrant Number
NIHHD-05753
NIHGM-20927
NSFBMS 75-07359
NIH Predoctoral FellowshipGM-00086
National Foundation - March of Dimes1-404
Damon Runyon-Walter Winchell Cancer FundDRG-19-F
American Cancer Society, California DivisionJ-340
Record Number:CaltechAUTHORS:20160418-085641040
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160418-085641040
Official Citation:Glenn A. Galau, William H. Klein, Roy J. Britten, Eric H. Davidson, Significance of rare mRNA sequences in liver, Archives of Biochemistry and Biophysics, Volume 179, Issue 2, 1977, Pages 584-599, ISSN 0003-9861, http://dx.doi.org/10.1016/0003-9861(77)90147-3. (http://www.sciencedirect.com/science/article/pii/0003986177901473)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66229
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:18 Apr 2016 16:23
Last Modified:13 Feb 2019 18:06

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