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Analysis of Thiols by Microchip Capillary Electrophoresis for In Situ Planetary Investigations

Mora, Maria F. and Stockton, Amanda M. and Willis, Peter A. (2015) Analysis of Thiols by Microchip Capillary Electrophoresis for In Situ Planetary Investigations. In: Microchip Capillary Electrophoresis Protocols. Methods in Molecular Biology. No.1274. Humana Press , New York, NY, pp. 43-52. ISBN 978-1-4939-2352-6.

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Microchip capillary electrophoresis with laser-induced fluorescence detection (μCE-LIF) enables sensitive analyses of a wide range of analytes employing small volumes of sample and reagent (nL to μL) on an instrument platform with minimal mass, volume, and power requirements. This technique has been used previously in the analysis of amino acids and other organic molecules of interest in the fields of astrobiology and planetary science. Here, we present a protocol for the analysis of thiols using μCE-LIF. This protocol utilizes Pacific Blue C5-maleimide for fluorescent derivatization of thiols, enabling limits of detection in the low nM range (1.4–15 nM). Separations are conducted in micellar electrokinetic chromatography mode with 25 mM sodium dodecyl sulfate in 15 mM tetraborate, pH 9.2. This method allows analysis of 12 thiols in less than 2 min following a labeling step of 2 h. A step-by-step protocol, including tips on microchip capillary electrophoresis, is described here.

Item Type:Book Section
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Additional Information:© 2015 Springer Science+Business Media New York.
Subject Keywords:Thiols; Microchip capillary electrophoresis; Laser-induced fluorescence; Pacific Blue C5-maleimide
Series Name:Methods in Molecular Biology
Issue or Number:1274
Record Number:CaltechAUTHORS:20160418-102832448
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66240
Deposited By: Tony Diaz
Deposited On:18 Apr 2016 18:27
Last Modified:03 Oct 2019 09:54

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