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The CDK-APC/C Oscillator Predominantly Entrains Periodic Cell-Cycle Transcription

Rahi, Sahand Jamal and Pecani, Kresti and Ondracka, Andrej and Oikonomou, Catherine M. and Cross, Frederick R. (2016) The CDK-APC/C Oscillator Predominantly Entrains Periodic Cell-Cycle Transcription. Cell, 165 (2). pp. 475-487. ISSN 0092-8674. PMCID PMC4826480. doi:10.1016/j.cell.2016.02.060.

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Throughout cell-cycle progression, the expression of multiple transcripts oscillate, and whether these are under the centralized control of the CDK-APC/C proteins or can be driven by a de-centralized transcription factor (TF) cascade is a fundamental question for understanding cell-cycle regulation. In budding yeast, we find that the transcription of nearly all genes, as assessed by RNA-seq or fluorescence microscopy in single cells, is dictated by CDK-APC/C. Three exceptional genes are transcribed in a pulsatile pattern in a variety of CDK-APC/C arrests. Pursuing one of these transcripts, the SIC1 inhibitor of B-type cyclins, we use a combination of mathematical modeling and experimentation to provide evidence that, counter-intuitively, Sic1 provides a failsafe mechanism promoting nuclear division when levels of mitotic cyclins are low.

Item Type:Article
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Oikonomou, Catherine M.0000-0003-2312-4746
Additional Information:© 2016 Elsevier Inc. Received: August 13, 2015; Revised: December 22, 2015; Accepted: February 22, 2016; Published: April 7, 2016. S.J.R. thanks Nahal Mansouri for insightful discussions and Aleeza Kazmi for help with image processing. The work was supported by NIH grant 5RO1-GM078153-07 to F.R.C., NRSA Training Grant CA009673-36A1, and Merck and Simons Foundation Postdoctoral Fellowships to S.J.R. Author Contributions: Conceptualization, S.J.R. and F.R.C.; Methodology, Writing, Analysis, S.J.R., K.P., and F.R.C.; Investigation, S.J.R., K.P., A.O., and C.O.
Funding AgencyGrant Number
NIH Predoctoral FellowshipCA009673-36A1
Merck FoundationUNSPECIFIED
Simons FoundationUNSPECIFIED
Issue or Number:2
PubMed Central ID:PMC4826480
Record Number:CaltechAUTHORS:20160418-152013494
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Official Citation:Sahand Jamal Rahi, Kresti Pecani, Andrej Ondracka, Catherine Oikonomou, Frederick R. Cross, The CDK-APC/C Oscillator Predominantly Entrains Periodic Cell-Cycle Transcription, Cell, Volume 165, Issue 2, 7 April 2016, Pages 475-487, ISSN 0092-8674, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66257
Deposited By: Tony Diaz
Deposited On:18 Apr 2016 23:44
Last Modified:10 Nov 2021 23:55

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