Strauss, James H. and Sinsheimer, Robert L. (1968) Initial Kinetics of Degradation of MS2 Ribonucleic Acid by Ribonuclease, Heat and Alkali and the Presence of Configurational Restraints in this Ribonucleic Acid. Journal of Molecular Biology, 34 (3). pp. 453-465. ISSN 0022-2836. doi:10.1016/0022-2836(68)90172-1. https://resolver.caltech.edu/CaltechAUTHORS:20160418-164908723
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Abstract
The initial kinetics of degradation of MS2 RNA by ribonuclease, heat and alkali were followed by the exponential decline in RNA infectivity. The turnover number of pancreatic ribonuclease is independent of RNA concentration between 10^(11) and 10^(15) RNA molecules per ml. Inactivation at high pH is almost proportional to the hydroxyl ion concentration between pH 11 and 12·3. The activation energy for thermal inactivation at neutral pH is 22 kcal./mole. MS2 RNA possesses no covalent configurational restraints, as shown by the correspondence between the decline in infectivity produced by ribonuclease and the production of RNA fragments, by the coincident sedimentation of the RNA infective material and the bulk RNA under a variety of conditions, and by the presence of only one component when the RNA is sedimented under denaturing conditions. The RNA chain does form intramolecular hydrogen bonds, which under certain conditions give rise to a multiplicity of discrete, infective components.
Item Type: | Article | |||||||||
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Additional Information: | © 1968 Elsevier. Received 20 November 1967, and in revised form 8 February 1968. We thank Mrs Gloria Davis for competent technical assistance with some of the infectivity assays. The material presented here is part of the thesis of James H. Strauss, Jr. submitted to the California Institute of Technology in partial fulfilment of the Ph.D. requirements, who was supported from U.S.P.H. training grant 2G-86. This research was supported in part by a grant GM 13554 from the U.S. Public Health Service. | |||||||||
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Issue or Number: | 3 | |||||||||
DOI: | 10.1016/0022-2836(68)90172-1 | |||||||||
Record Number: | CaltechAUTHORS:20160418-164908723 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160418-164908723 | |||||||||
Official Citation: | James H. Strauss, Robert L. Sinsheimer, Initial kinetics of degradation of MS2 ribonucleic acid by ribonuclease, heat and alkali and the presence of configurational restraints in this ribonucleic acid, Journal of Molecular Biology, Volume 34, Issue 3, 1968, Pages 453-465, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(68)90172-1. (http://www.sciencedirect.com/science/article/pii/0022283668901721) | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 66264 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Donna Wrublewski | |||||||||
Deposited On: | 19 Apr 2016 00:05 | |||||||||
Last Modified: | 10 Nov 2021 23:55 |
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