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Glycopeptides of the membrane glycoprotein of Sindbis virus

Burge, Boyce W. and Strauss, James H., Jr. (1970) Glycopeptides of the membrane glycoprotein of Sindbis virus. Journal of Molecular Biology, 47 (3). pp. 449-466. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160418-165958363

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Abstract

The membrane of the Sindbis virus (an arbovirus) is structurally simple; it is composed of lipids and a single glycoprotein of molecular weight estimated at 53,000 daltons. Digestion of the membrane glycoprotein with pronase yields a set of glycopeptides (largely polysaccharide) that can be characterized by molecular sieving. From virus grown in chick fibroblasts, three principal glycopeptide peaks were identified, with molecular weights of 3200, 2800 and 1800 daltons. The smallest glycopeptide, estimated to contain nine sugar residues, contains only mannose and glucosamine, while the larger glycopeptides are composed of mannose, glucosamine, galactose, fucose and, presumably, sialic acid. There are 3 or 4 moles of glycopeptide per mole of membrane protein. Glycopeptides from virus grown in hamster cells have a somewhat different size distribution, but seem grossly similar to the glycopeptides of chick-grown Sindbis virus. In the infected cell, 75% of newly synthesized glycopeptide material is found in a membrane fraction; these glycopeptides are of the same range of molecular weights as those found in virus particles, but are shifted to a smaller average molecular weight. It is not yet possible to determine whether synthesis of the carbohydrate moiety of the virus membrane glycoprotein is specified by viral enzymes, host enzymes or some combination of the two.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/0022-2836(70)90314-1DOIArticle
http://www.sciencedirect.com/science/article/pii/0022283670903141PublisherArticle
Additional Information:© 1970 Elsevier. Received 18 July 1969. Dr James Darnell Jr, played a seminal role in this investigation by suggesting that the Sindbis membrane protein might be a glycoprotein. He also contributed editorial skills in the construction of this paper. R. Tushinski and J. Morgenstern provided very competent technical assistance. This work was supported by grants from the National Institutes of Health (CA 07861-05), the National Science Foundation (GB 4565), and the Health Research Council of the City of New York.
Funders:
Funding AgencyGrant Number
NIHCA 07861-05
NSFGB 4565
Health Research Council of the City of New YorkUNSPECIFIED
Issue or Number:3
Record Number:CaltechAUTHORS:20160418-165958363
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160418-165958363
Official Citation:Boyce W. Burge, James H. Strauss, Glycopeptides of the membrane glycoprotein of Sindbis virus, Journal of Molecular Biology, Volume 47, Issue 3, 1970, Pages 449-466, ISSN 0022-2836, http://dx.doi.org/10.1016/0022-2836(70)90314-1. (http://www.sciencedirect.com/science/article/pii/0022283670903141)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66265
Collection:CaltechAUTHORS
Deposited By: Donna Wrublewski
Deposited On:19 Apr 2016 00:07
Last Modified:03 Oct 2019 09:55

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