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Benzyl butyl phthalate promotes adipogenesis in 3T3-L1 preadipocytes: A High Content Cellomics and metabolomic analysis

Yin, Lei and Yu, Kevin Shengyang and Lu, Kun and Yu, Xiaozhong (2016) Benzyl butyl phthalate promotes adipogenesis in 3T3-L1 preadipocytes: A High Content Cellomics and metabolomic analysis. Toxicology in Vitro, 32 . pp. 297-309. ISSN 0887-2333. http://resolver.caltech.edu/CaltechAUTHORS:20160422-131139363

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Abstract

Benzyl butyl phthalate (BBP) has been known to induce developmental and reproductive toxicity. However, its association with dysregulation of adipogenesis has been poorly investigated. The present study aimed to examine the effect of BBP on the adipogenesis, and to elucidate the underlying mechanisms using the 3T3-L1 cells. The capacity of BBP to promote adipogenesis was evaluated by multiple staining approaches combined with a High Content Cellomics analysis. The dynamic changes of adipogenic regulatory genes and proteins were examined, and the metabolite profile was identified using GC/MC based metabolomic analysis. The High Content analysis showed BBP in contrast with Bisphenol A (BPA), a known environmental obesogen, increased lipid droplet accumulation in a similar dose-dependent manner. However, the size of the lipid droplets in BBP-treated cells was significantly larger than those in cells treated with BPA. BBP significantly induced mRNA expression of transcriptional factors C/EBPα and PPARγ, their downstream genes, and numerous adipogenic proteins in a dose and time-dependent manner. Furthermore, GC/MC metabolomic analysis revealed that BBP exposure perturbed the metabolic profiles that are associated with glyceroneogenesis and fatty acid synthesis. Altogether, our current study clearly demonstrates that BBP promoted the differentiation of 3T3-L1 through the activation of the adipogenic pathway and metabolic disturbance.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.tiv.2016.01.010DOIArticle
http://www.sciencedirect.com/science/article/pii/S0887233316300108PublisherArticle
Additional Information:© 2016 Elsevier B.V. Received 8 September 2015, Revised 12 January 2016, Accepted 20 January 2016, Available online 25 January 2016.
Subject Keywords:Benzyl butyl phthalate; Adipogenesis; High Content Cellomics analysis (HCA); Gene expression; Metabolomic analysis
Record Number:CaltechAUTHORS:20160422-131139363
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160422-131139363
Official Citation:Lei Yin, Kevin Shengyang Yu, Kun Lu, Xiaozhong Yu, Benzyl butyl phthalate promotes adipogenesis in 3T3-L1 preadipocytes: A High Content Cellomics and metabolomic analysis, Toxicology in Vitro, Volume 32, April 2016, Pages 297-309, ISSN 0887-2333, http://dx.doi.org/10.1016/j.tiv.2016.01.010. (http://www.sciencedirect.com/science/article/pii/S0887233316300108)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66414
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Apr 2016 20:16
Last Modified:22 Apr 2016 20:16

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