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Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic Poly(I:C)

Chow, Ke-Huan and Yan, Zihao and Wu, Wei-Li (2016) Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic Poly(I:C). Journal of Visualized Experiments (109). Art. No. 53643. ISSN 1940-087X .

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Maternal immune activation (MIA) model is increasingly well appreciated as a rodent model for the environmental risk factor of various psychiatric disorders. Numerous studies have demonstrated that MIA model is able to show face, construct, and predictive validity that are relevant to autism and schizophrenia. To model MIA, investigators often use viral mimic polyinosinic:polycytidylic acid (poly(I:C)) to activate the immune system in pregnant rodents. Generally, the offspring from immune activated dam exhibit behavioral abnormalities and physiological alterations that are associated with autism and schizophrenia. However, poly(I:C) injection with different dosages and at different time points could lead to different outcomes by perturbing brain development at different stages. Here we provide a detailed method of inducing MIA by intraperitoneal (i.p.) injection of 20 mg/kg poly(I:C) at mid-gestational embryonic 12.5 days (E12.5). This method has been shown to induce acute inflammatory response in the maternal-placental-fetal axis, which ultimately results in the brain perturbations and behavioral phenotypes that are associated with autism and schizophrenia.

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Additional Information:© 2016 Journal of Visualized Experiments. Date Published: 3/25/2016. We would like to honor the late Dr. Paul H. Patterson for his contributions to the progress of MIA model, autism, and schizophrenia research. We acknowledge Sarkis K. Mazmanian for his great support on this protocol; Ruben M. Bayon, Yvette Garcia-Flores, Karen C. Lencioni, and Leslie A. Neumann for administrative assistance; Ali Khoshnan and Jan C. Ko for the assistance on filming; Elaine Y. Hsiao and Natalia Malkova for their advice on MIA induction; Jeffrey S. Cochrane, Joaquin Gutierrez, Kwan F. Lee, Jaime Rodriguez, Lorena C. Sandoval, and Natalie A. Verduzco for their expert animal husbandry. This work was supported by the NIH Conte Center Award (NIH 5P50MH086383-04, to Paul H. Patterson); Autism Speaks (#7670, to Paul H. Patterson); Simons Foundation (#322839, to Sarkis K. Mazmanian); NIH Training Grant (NIH/NRSA T32GM07616 to K.-H.C.); Caltech Summer Undergraduate Research Fellowship (SURF) (to Z.Y.); Amgen Scholars Program at Caltech (to Z.Y.); and Postdoctoral Fellowship from National Science Council, Taiwan (NSC 101-2917-I-564-039, to W.-L.W.). The authors have nothing to disclose.
Funding AgencyGrant Number
Autism Speaks7670
Simons Foundation322839
NIH Predoctoral FellowshipT32GM07616
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
National Science Council (Taipei)NSC 101-2917-I-564-039
Subject Keywords:Immunology, Issue 109, Maternal infection, Maternal immune activation (MIA), Polyinosinic:polycytidylic acid (Poly(I:C)), Embryonic 12.5 days (E12.5), Interleukin-6 (IL-6), Maternal-placental-fetal axis, Cerebellar Purkinje cells, Hindbrain, Autism, Schizophrenia, Behavioral abnormalities
Record Number:CaltechAUTHORS:20160425-081243284
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Official Citation:Chow, K. H., Yan, Z., Wu, W. L. Induction of Maternal Immune Activation in Mice at Mid-gestation Stage with Viral Mimic Poly(I:C). J. Vis. Exp. (109), e53643, doi:10.3791/53643 (2016)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66435
Deposited By: Tony Diaz
Deposited On:02 May 2016 23:14
Last Modified:02 May 2016 23:14

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