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Identification and differential production of ubiquinone-8 in the bacterial predator Bdellovibrio bacteriovorus

Spain, Eileen M. and Núñez, Megan E. and Kim, Hyeong-Jin and Taylor, Ryan J. and Thomas, Nicholas and Wengen, Michael B. and Dalleska, Nathan F. and Bromley, Joseph P. and Schermerhorn, Kimberly H. and Ferguson, Megan A. (2016) Identification and differential production of ubiquinone-8 in the bacterial predator Bdellovibrio bacteriovorus. Research in Microbiology, 167 (5). pp. 413-423. ISSN 0923-2508 . http://resolver.caltech.edu/CaltechAUTHORS:20160426-112826025

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Abstract

Bdellovibrio bacteriovorus 109J, a predatory bacterium with potential as a bacterial control agent, can exist in several lifestyles that differ both in predatory capacity and color. We determined that levels of ubiquinone-8 contribute to the distinctive but variable yellow color of different types of Bdellovibrio cells. Steady-state ubiquinone-8 concentrations did not differ markedly between conventional predatory and host-independent B. bacteriovorus despite upregulation of a suite of ubiquinone-8 synthesis genes in host-independent cells. In contrast, in spatially organized B. bacteriovorus films, the yellow inner regions contain significantly higher ubiquinone-8 concentrations than the off-white outer regions. Correspondingly, RT-PCR analysis reveals that the inner region, previously shown to consist primarily of active predators, clearly expresses two ubiquinone biosynthesis genes, while the outer region, composed mainly of quiescent or stalled bdelloplasts, expresses those genes weakly or not at all. Moreover, B. bacteriovorus cells in the inner region of week-old interfacial films, which are phenotypically attack-phase, have much higher UQ8 levels than regular attack-phase bdellovibrios, most likely because their “trapped” state prevents a high expenditure of energy to power flagellar motion.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.resmic.2016.04.003DOIArticle
http://www.sciencedirect.com/science/article/pii/S0923250816300055PublisherArticle
ORCID:
AuthorORCID
Thomas, Nicholas0000-0002-6882-9822
Dalleska, Nathan F.0000-0002-2059-1587
Additional Information:© 2016 Published by Elsevier Masson SAS on behalf of Institut Pasteur. Received 6 August 2015; accepted 2 April 2016. NFD, MAF, and EMS are grateful to Dr. John Greaves of UC Irvine for his assistance with high-resolution mass spectrometry. EMS and MAF thank Weidong Wang (Occidental College) for advice on NMR studies. We also thank the Research, Scholarship and Creative Activities program at SUNY New Paltz, the Undergraduate Research Center at Occidental College, and Occidental's Biochemistry program. This work was supported by NSF (CHE-0910578) as well as a grant to Occidental College from HHMI through the Undergraduate and Graduate Science Education Programs. The authors declare that they have no conflicts of interest with the contents of this article.
Funders:
Funding AgencyGrant Number
NSFCHE-0910578
Occidental CollegeUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:Bacterial metabolism; Electron transport; Gene expression; Phase variation; Predator-prey interactions
Record Number:CaltechAUTHORS:20160426-112826025
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160426-112826025
Official Citation:Eileen M. Spain, Megan E. Núñez, Hyeong-Jin Kim, Ryan J. Taylor, Nicholas Thomas, Michael B. Wengen, Nathan F. Dalleska, Joseph P. Bromley, Kimberly H. Schermerhorn, Megan A. Ferguson, Identification and differential production of ubiquinone-8 in the bacterial predator Bdellovibrio bacteriovorus, Research in Microbiology, Volume 167, Issue 5, June 2016, Pages 413-423, ISSN 0923-2508, http://dx.doi.org/10.1016/j.resmic.2016.04.003. (http://www.sciencedirect.com/science/article/pii/S0923250816300055)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66484
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:27 Apr 2016 03:40
Last Modified:22 Mar 2019 22:46

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