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Development of prokaryotic cell-free systems for synthetic biology

Chiao, Abel C. and Murray, Richard M. and Sun, Zachary Z. (2016) Development of prokaryotic cell-free systems for synthetic biology. . (Unpublished)

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Prokaryotic cell-free systems are currently heavily used for the production of protein that can be otherwise challenging to produce in cells. However, historically cell-free systems were used to explore natural phenomena before the advent of genetic modification and transformation technology. Recently, synthetic biology has seen a resurgence of this historical use of cell-free systems as a prototyping tool of synthetic and natural genetic circuits. For these cell-free systems to be effective prototyping tools, an understanding of cell-free system mechanics must be established that is not purely protein-expression driven. Here we discuss the development of E. coli-based cell-free systems, with an emphasis on documenting published extract and energy preparation methods into a uniform format. We also discuss additional considerations when applying cell-free systems to synthetic biology.

Item Type:Report or Paper (Technical Report)
Related URLs:
URLURL TypeDescription Paper
Murray, Richard M.0000-0002-5785-7481
Sun, Zachary Z.0000-0002-9425-2924
Additional Information:© 2016. The copyright holder for this preprint is the author/funder. It is made available under a CC-BY-NC-ND 4.0 International license. bioRxiv preprint first posted online April 15, 2016. NOTE: This is a technical report for future inclusion in work pending submission, review, and publication. Therefore, this work has not been peer-reviewed and is presented asis. The authors declare a conflict of interest: ACC, RMM, ZSS hold ownership in Synvitrobio, Inc. The work presented here was funded off of a DARPA SBIR to Synvitrobio, Inc. (ACC, ZSS), contract No: W911NF-16-P-0003, and a Caltech Grubstake Grant (ACC, RMM, ZSS). The views and conclusions contained in this document are those of the authors and should not be interpreted as representing officially policies, either expressly or implied, of the Defense Advanced Research Projects Agency or the U.S. Government.
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)W911NF-16-P-0003
Caltech Grubstake GrantUNSPECIFIED
Subject Keywords:Synthetic biology, industrial biotechnology; Cell-free expression systems, biosynthesis, protein expression
Record Number:CaltechAUTHORS:20160502-104806241
Persistent URL:
Official Citation:Development of prokaryotic cell-free systems for synthetic biology Abel C Chiao, Richard M Murray, Zachary Z Sun bioRxiv doi:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66583
Deposited By: Ruth Sustaita
Deposited On:02 May 2016 19:01
Last Modified:11 Nov 2021 00:00

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