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Footprinting with MPE•Fe(II). Complementary-strand analyses of distamycin- and actinomycin-binding sites on heterogeneous DNA

Van Dyke, M. W. and Dervan, P. B. (1983) Footprinting with MPE•Fe(II). Complementary-strand analyses of distamycin- and actinomycin-binding sites on heterogeneous DNA. Cold Spring Harbor Symposia on Quantitative Biology, 47 . pp. 347-353. ISSN 0091-7451. https://resolver.caltech.edu/CaltechAUTHORS:20160505-094707943

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Abstract

We recently reported a direct technique for determining the binding sites of small molecules on naturally occurring heterogeneous DNA (Van Dyke et al. 1982). Methidiumpropyl-EDTA·Fe(II) (MPE·Fe[II]) (Hertzberg and Dervan 1982) cleaves double-helical DNA with low sequence-specificity (Van Dyke et al. 1982). Using a combination of MPE·Fe(II) partial cleavage of drug-protected DNA fragments and Maxam-Gilbert sequencing methods, we determined the drug-protected sites on one strand of a double-helical fragment from pBR322 for the intercalator actinomycin D (Goldberg et al. 1962; Muller and Crothers 1968; Wells and Larson 1970; Sobell 1973; Krugh 1981; Patel et al. 1981; Takusagawa et al. 1982) and the minor-groove binders netropsin and distamycin A (Luck et al. 1974; Wartell et al. 1974; Zimmer 1975; Berman et al. 1979; Krylov et al. 1979). Netropsin and distamycin A gave identical DNA-cleavage inhibition patterns or footprints in regions rich in dA·dT base pairs. Actinomycin D afforded a completely different footprint...


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1101/SQB.1983.047.01.040DOIArticle
http://symposium.cshlp.org/content/47/347.extractPublisherArticle
ORCID:
AuthorORCID
Dervan, P. B.0000-0001-8852-7306
Additional Information:© 1983 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). We are grateful to the National Institutes of Health for grant support (GM-27681) and a National Research Service Award (GM-07616) to M.W.V.
Funders:
Funding AgencyGrant Number
NIHGM-27681
NIH Predoctoral FellowshipGM-07616
Record Number:CaltechAUTHORS:20160505-094707943
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160505-094707943
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66677
Collection:CaltechAUTHORS
Deposited By: George Porter
Deposited On:09 May 2016 17:58
Last Modified:03 Oct 2019 09:58

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