Van Dyke, M. W. and Dervan, P. B. (1983) Footprinting with MPE•Fe(II). Complementary-strand analyses of distamycin- and actinomycin-binding sites on heterogeneous DNA. Cold Spring Harbor Symposia on Quantitative Biology, 47 . pp. 347-353. ISSN 0091-7451. doi:10.1101/SQB.1983.047.01.040. https://resolver.caltech.edu/CaltechAUTHORS:20160505-094707943
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Abstract
We recently reported a direct technique for determining the binding sites of small molecules on naturally occurring heterogeneous DNA (Van Dyke et al. 1982). Methidiumpropyl-EDTA·Fe(II) (MPE·Fe[II]) (Hertzberg and Dervan 1982) cleaves double-helical DNA with low sequence-specificity (Van Dyke et al. 1982). Using a combination of MPE·Fe(II) partial cleavage of drug-protected DNA fragments and Maxam-Gilbert sequencing methods, we determined the drug-protected sites on one strand of a double-helical fragment from pBR322 for the intercalator actinomycin D (Goldberg et al. 1962; Muller and Crothers 1968; Wells and Larson 1970; Sobell 1973; Krugh 1981; Patel et al. 1981; Takusagawa et al. 1982) and the minor-groove binders netropsin and distamycin A (Luck et al. 1974; Wartell et al. 1974; Zimmer 1975; Berman et al. 1979; Krylov et al. 1979). Netropsin and distamycin A gave identical DNA-cleavage inhibition patterns or footprints in regions rich in dA·dT base pairs. Actinomycin D afforded a completely different footprint...
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Additional Information: | © 1983 Cold Spring Harbor Laboratory Press. The Authors acknowledge that six months after the full-issue publication date, the Article will be distributed under a Creative Commons CC-BY-NC License (Attribution-NonCommercial 4.0 International License, http://creativecommons.org/licenses/by-nc/4.0/). We are grateful to the National Institutes of Health for grant support (GM-27681) and a National Research Service Award (GM-07616) to M.W.V. | |||||||||
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DOI: | 10.1101/SQB.1983.047.01.040 | |||||||||
Record Number: | CaltechAUTHORS:20160505-094707943 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160505-094707943 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 66677 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | George Porter | |||||||||
Deposited On: | 09 May 2016 17:58 | |||||||||
Last Modified: | 11 Nov 2021 00:01 |
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