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Programming multiple protein patterns on a single DNA nanostructure

Cohen, Justin D. and Sadowski, John P. and Dervan, Peter B. (2008) Programming multiple protein patterns on a single DNA nanostructure. Journal of the American Chemical Society, 130 (2). pp. 402-403. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20160509-104242378

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Abstract

The ability to create assemblies of proteins with spacing on the nanometer scale has important implications for proteomics, biodetection, and self-assembly. Structural DNA nanotechnology has led to the creation of a variety of nanostructures which should be capable of serving as an addressable template for the creation of complex molecular assemblies. The goal of such systems is to be able to position proteins or other components in distinct patterns with precise spacing. These systems take advantage of the well-defined structure and spacing of DNA and use these properties to act as a template for secondary components in a bottom-up approach toward self-assembly. Previous work in this area has primarily focused on the use of chemical or structural modifications of the DNA template in order to attach or recruit proteins or nanoparticles. We have recently shown that a single polyamide-biotin conjugate is capable of binding to a DX array made from two tiles without any modification of the target DNA.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0772400DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja0772400PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja0772400PublisherSupporting Information
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 2008 American Chemical Society. Received September 18, 2007. We thank Prof. Erik Winfree and Dr. Sung Ha Park for useful discussions and assistance with the AFM experiments. We are grateful for fellowship support (J.D.C.) from the Ralph M. Parsons Foundation.
Funders:
Funding AgencyGrant Number
Ralph M. Parsons FoundationUNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20160509-104242378
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160509-104242378
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66739
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:17 May 2016 19:59
Last Modified:26 Nov 2019 11:15

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