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Fluorescent Sequence-Specific dsDNA Binding Oligomers

Chenoweth, David M. and Viger, Anne and Dervan, Peter B. (2007) Fluorescent Sequence-Specific dsDNA Binding Oligomers. Journal of the American Chemical Society, 129 (8). pp. 2216-2217. ISSN 0002-7863. PMCID PMC3066654. doi:10.1021/ja0682576. https://resolver.caltech.edu/CaltechAUTHORS:20160509-110448610

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Abstract

Sequence-specific detection methods for double-stranded DNA that obviate the need for denaturation would provide a powerful tool for bioorganic chemistry and genetics. As part of a sustained effort to develop sequence-specific fluorescent DNA detection methods, two programmable oligomers have been synthesized which target their respective sequences 5‘-WTACGW-3‘ and 5‘-WGGGGW-3‘ (W = A or T). The two oligomers were found to fluoresce weakly in the absence of DNA but showed significant fluorescence enhancement by the addition of match DNA. The fluorescence is shown to increase in a concentration-dependent manner, and the intensity varies depending on the number of mismatch sites incorporated into the DNA hairpins. This new class of oligomers provides a method to detect DNA sequences without denaturation and in the absence of conjugation to a dye molecule. This is a first step toward sequence-specific DNA-binding molecules containing a fluorescent switch integrated as part of the recognition modules.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja0682576DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja0682576PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/ja0682576PublisherSupporting Information
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3066654/PubMed CentralArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 2007 American Chemical Society. Received November 17, 2006. Publication Date (Web): February 6, 2007. We thank Bert T. Lai in the Beckman Institute Laser Resource Center for use of the fluorimeter. We are grateful to The National Institutes of Health for grant support and the Swiss National Science Foundation for a fellowship to A.V.
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
Swiss National Science Foundation (SNSF)UNSPECIFIED
Issue or Number:8
PubMed Central ID:PMC3066654
DOI:10.1021/ja0682576
Record Number:CaltechAUTHORS:20160509-110448610
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160509-110448610
Official Citation:Fluorescent Sequence-Specific dsDNA Binding Oligomers David M. Chenoweth, Anne Viger, and Peter B. Dervan Journal of the American Chemical Society 2007 129 (8), 2216-2217 DOI: 10.1021/ja0682576
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66752
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:17 May 2016 21:13
Last Modified:11 Nov 2021 00:02

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