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DNA-templated dimerization of hairpin polyamides

Poulin-Kerstien, Adam T. and Dervan, Peter B. (2003) DNA-templated dimerization of hairpin polyamides. Journal of the American Chemical Society, 125 (51). pp. 15811-21. ISSN 0002-7863. doi:10.1021/ja030494a.

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Double-helical DNA accelerates the rate of ligation of two six-ring hairpin polyamides which bind adjacent sites in the minor groove via a 1,3-dipolar cycloaddition to form a tandem dimer. The rate of the templated reaction is dependent on DNA sequence as well as on the distance between the hairpin-binding sites. The tandem dimer product of the DNA-templated reaction has improved binding properties with respect to the smaller hairpin fragments. Since cell and nuclear uptake of DNA-binding polyamides will likely be dependent on size, this is a minimum first step toward the design of self-assembling small gene-regulating fragments to produce molecules of increasing complexity with more specific genomic targeting capabilities.

Item Type:Article
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URLURL TypeDescription Information
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 2003 American Chemical Society. Publication Date (Web): November 25, 2003. Mass spectral analyses were performed in the Mass Spectrometry Laboratory of the Division of Chemistry and Chemical Engineering of Caltech, supported in part by the NSF MRSEC program. We thank the National Institutes of Health for generous research support and for a predoctoral training grant to A.T.K.
Funding AgencyGrant Number
NIH Predoctoral FellowshipUNSPECIFIED
Issue or Number:51
Record Number:CaltechAUTHORS:20160510-080144724
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Official Citation:DNA-Templated Dimerization of Hairpin Polyamides Adam T. Poulin-Kerstien and Peter B. Dervan Journal of the American Chemical Society 2003 125 (51), 15811-15821 DOI: 10.1021/ja030494a
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66796
Deposited By: Victoria Brennan
Deposited On:17 May 2016 20:24
Last Modified:11 Nov 2021 00:03

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