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Design of artificial transcriptional activators with rigid poly-L-proline linkers

Arora, Paramjit S. and Ansari, Aseem Z. and Best, Timothy P. and Ptashne, Mark and Dervan, Peter B. (2002) Design of artificial transcriptional activators with rigid poly-L-proline linkers. Journal of the American Chemical Society, 124 (44). pp. 13067-13071. ISSN 0002-7863. doi:10.1021/ja0208355.

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Typical eukaryotic transcriptional activators are composed of distinct functional domains, including a DNA binding domain and an activating domain. Artificial transcription factors have been designed wherein the DNA binding domain is a minor groove DNA binding hairpin polyamide linked by a flexible tether to short activating peptides, typically 16-20 residues in size. In this study, the linker between the polyamide and the peptide was altered in an incremental fashion using rigid oligoproline "molecular rulers" in the 18-45 A length range. We find that there is an optimal linker length which separates the DNA and the activation region for transcription activation.

Item Type:Article
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URLURL TypeDescription Information
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 2002 American Chemical Society. Received June 12, 2002; Publication Date (Web): October 15, 2002. We are grateful to the National Institutes of Health for research support, the American Cancer Society for a postdoctoral fellowship to P.S.A., and the Helen Hay Whitney Foundation for a fellowship to A.Z.A.
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American Cancer SocietyUNSPECIFIED
Helen Hay Whitney FoundationUNSPECIFIED
Issue or Number:44
Record Number:CaltechAUTHORS:20160510-081402336
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Official Citation:Design of Artificial Transcriptional Activators with Rigid Poly-l-proline Linkers Paramjit S. Arora, Aseem Z. Ansari, Timothy P. Best, Mark Ptashne, and Peter B. Dervan Journal of the American Chemical Society 2002 124 (44), 13067-13071 DOI: 10.1021/ja0208355
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66812
Deposited By: Victoria Brennan
Deposited On:18 May 2016 18:48
Last Modified:11 Nov 2021 00:03

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