Zhan, Zheng-Yun J. and Dervan, Peter B. (2000) Alternative Heterocycles for DNA Recognition: A 3-Pyrazole/Pyrrole Pair Specifies for G·C Base Pairs. Bioorganic and Medicinal Chemistry, 8 (10). pp. 2467-2474. ISSN 0968-0896. doi:10.1016/S0968-0896(00)00182-6. https://resolver.caltech.edu/CaltechAUTHORS:20160510-091108944
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160510-091108944
Abstract
Synthetic ligands comprising three aromatic amino acids, pyrrole (Py), imidazole (Im), and hydroxypyrrole (Hp), specifically recognize predetermined sequences as side-by-side pairs in the minor groove of DNA. To expand the repertoire of aromatic rings that may be utilized for minor groove recognition, three five-membered heterocyclic rings, 3-pyrazolecarboxylic acid (3-Pz), 4-pyrazolecarboxylic acid (4-Pz), and furan-2-carboxylic acid (Fr), were examined at the N-terminus of eight-ring hairpin polyamide ligands. The DNA binding properties of 3-Pz, 4-Pz, and Fr each paired with Py were studied by quantitative DNase I footprinting titrations on a 283 bp DNA restriction fragment containing four 6-bp binding sites 5′-A TNCCTAA-3′ (N=G, C, A, or T; 6-bp polyamide binding site is underlined). The pair 3-Pz/Py has increased binding affinity and sequence specificity for G·C bp compared with Im/Py.
Item Type: | Article | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| |||||||||
ORCID: |
| |||||||||
Additional Information: | © 2000 Elsevier Science Ltd. Received 3 April 2000; accepted 16 June 2000. We are grateful to the National Institutes of Health (GM-27681) for research support. | |||||||||
Funders: |
| |||||||||
Issue or Number: | 10 | |||||||||
DOI: | 10.1016/S0968-0896(00)00182-6 | |||||||||
Record Number: | CaltechAUTHORS:20160510-091108944 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-091108944 | |||||||||
Official Citation: | Zheng-Yun J Zhan, Peter B Dervan, alternative heterocycles for dna recognition: a 3-pyrazole/pyrrole pair specifies for g·c base pairs, Bioorganic & Medicinal Chemistry, Volume 8, Issue 10, October 2000, Pages 2467-2474, ISSN 0968-0896, http://dx.doi.org/10.1016/S0968-0896(00)00182-6. (http://www.sciencedirect.com/science/article/pii/S0968089600001826) | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 66846 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Victoria Brennan | |||||||||
Deposited On: | 18 May 2016 20:44 | |||||||||
Last Modified: | 11 Nov 2021 00:22 |
Repository Staff Only: item control page