Trauger, John W. and Baird, Eldon E. and Dervan, Peter B. (1998) Recognition of 16 base pairs in the minor groove of DNA by a pyrrole-imidazole polyamide dimer. Journal of the American Chemical Society, 120 (14). pp. 3534-3535. ISSN 0002-7863. doi:10.1021/ja9800378. https://resolver.caltech.edu/CaltechAUTHORS:20160510-093520387
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Abstract
Cell-permeable small molecules that bind predetermined DNA sequences with affinities and specificities comparable to those of natural DNA-binding proteins have the potential to regulate the expression of specific genes. Recently, an eight-ring hairpin pyrrole-imidazole (Py-Im) polyamide which binds six base pairs of DNA was shown to inhibit transcription of a specific gene in cell culture. Polyamides recognizing longer DNA sequences should provide more specific biological activity. To specify a single site within the 3 billion base pair human genome, ligands which specifically recognize 15-16 base pairs are necessary. For this reason, recognition of 16 base pairs represents a milestone in the development of chemical approaches to DNA recognition. We examine here the affinity and specificity of a Py-Im polyamide dimer which targets 16 contiguous base pairs in the minor groove of DNA.
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Additional Information: | © 1998 American Chemical Society. Received January 5, 1998. Publication Date (Web): March 28, 1998. We are grateful to the National Institutes of Health (Grant GM-51747) for research support, to the National Science Foundation and the Ralph M. Parsons Foundation for predoctoral fellowships to J.W.T., and to the Howard Hughes Medical Institute for a predoctoral fellowship to E.E.B. | ||||||||||
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Issue or Number: | 14 | ||||||||||
DOI: | 10.1021/ja9800378 | ||||||||||
Record Number: | CaltechAUTHORS:20160510-093520387 | ||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-093520387 | ||||||||||
Official Citation: | Recognition of 16 Base Pairs in the Minor Groove of DNA by a Pyrrole−Imidazole Polyamide Dimer John W. Trauger, Eldon E. Baird, and Peter B. Dervan Journal of the American Chemical Society 1998 120 (14), 3534-3535 DOI: 10.1021/ja9800378 | ||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
ID Code: | 66862 | ||||||||||
Collection: | CaltechAUTHORS | ||||||||||
Deposited By: | Victoria Brennan | ||||||||||
Deposited On: | 11 May 2016 00:15 | ||||||||||
Last Modified: | 11 Nov 2021 00:23 |
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