Bachelin, M. and Hessler, G. and Kurz, G. and Hacia, J. G. and Dervan, P. B. and Kessler, H. (1998) Structure of a stereoregular phosphorothioate DNA/RNA duplex. Nature Structural Biology, 5 (4). pp. 271-276. ISSN 1072-8368. doi:10.1038/nsb0498-271. https://resolver.caltech.edu/CaltechAUTHORS:20160510-094622267
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160510-094622267
Abstract
In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-R_p)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.
Item Type: | Article | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Related URLs: |
| |||||||||
ORCID: |
| |||||||||
Additional Information: | © 1998 Nature Publishing Group. Received 17 October 1997; accepted 30 January 1998. We are grateful to the NIH for research support and the HHMI for a predoctoral fellowship to J. G. H. | |||||||||
Funders: |
| |||||||||
Issue or Number: | 4 | |||||||||
DOI: | 10.1038/nsb0498-271 | |||||||||
Record Number: | CaltechAUTHORS:20160510-094622267 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-094622267 | |||||||||
Official Citation: | Structure of a stereoregular phosphorothioate DNA/RNA duplex M. Bachelin, G. Hessler, G. Kurz, J. G. Hacia, P. B. Dervan & H. Kessler Nature Structural Biology 5, 271 - 276 (1998) doi:10.1038/nsb0498-271 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 66867 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Victoria Brennan | |||||||||
Deposited On: | 17 May 2016 22:53 | |||||||||
Last Modified: | 11 Nov 2021 00:23 |
Repository Staff Only: item control page