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Structure of a stereoregular phosphorothioate DNA/RNA duplex

Bachelin, M. and Hessler, G. and Kurz, G. and Hacia, J. G. and Dervan, P. B. and Kessler, H. (1998) Structure of a stereoregular phosphorothioate DNA/RNA duplex. Nature Structural Biology, 5 (4). pp. 271-276. ISSN 1072-8368. doi:10.1038/nsb0498-271.

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In this work, we present the first NMR solution structure of a DNA/RNA hybrid containing stereoregular Rp-phosphorothioate modifications of all DNA backbone linkages. The complex of the enzymatically synthesized phosphorothioate DNA octamer (all-R_p)-d(GCGTCAGG) and its complementary RNA r(CCUGACGC) was found to adopt an overall conformation within the A-form family. Most helical parameters and the sugar puckers of the DNA strand assume values intermediate between A- and B-form. The close structural similarity with the unmodified DNA/RNA hybrid of the same sequence may explain why both the natural and the sulfur-substituted complex can be recognized and digested by ribonuclease H.

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URLURL TypeDescription DOIArticle
Dervan, P. B.0000-0001-8852-7306
Additional Information:© 1998 Nature Publishing Group. Received 17 October 1997; accepted 30 January 1998. We are grateful to the NIH for research support and the HHMI for a predoctoral fellowship to J. G. H.
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Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:4
Record Number:CaltechAUTHORS:20160510-094622267
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Official Citation:Structure of a stereoregular phosphorothioate DNA/RNA duplex M. Bachelin, G. Hessler, G. Kurz, J. G. Hacia, P. B. Dervan & H. Kessler Nature Structural Biology 5, 271 - 276 (1998) doi:10.1038/nsb0498-271
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66867
Deposited By: Victoria Brennan
Deposited On:17 May 2016 22:53
Last Modified:11 Nov 2021 00:23

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