Szewczyk, Jason W. and Baird, Eldon E. and Dervan, Peter B. (1996) Cooperative triple-helix formation via a minor groove dimerization domain. Journal of the American Chemical Society, 118 (28). pp. 6778-6779. ISSN 0002-7863. doi:10.1021/ja960831y. https://resolver.caltech.edu/CaltechAUTHORS:20160510-102731520
Full text is not posted in this repository. Consult Related URLs below.
Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160510-102731520
Abstract
Cooperative interactions between DNA-binding proteins are critical to the regulation of gene expression. When cooperative interactions occur between two oligonucleotides bound at neighboring sites on double-helical DNA, the specific binding of each oligonucleotide is enhanced. Pyrimidine oligodeoxyribonucleotides 11 bases in length bind cooperatively to abutting sites on double-helical DNA by triple-helix formation. Cooperative interactions up to 2.3 kcal mol^(-1) were obtained by addition of dimerization domains to oligonucleotides such as those capable of forming a short Watson−Crick helix. The designed polyamide ImPyPy binds sequence specifically to the minor groove of DNA at 5‘-(T,A)G(T,A)C(T,A)-3‘ sites as a cooperative side-by-side antiparallel homodimer. We report here that a pyrrole−imidazole polyamide oligonucleotide can bind cooperatively to a double-stranded DNA template via a sequence specific polyamide dimerization domain.
| Item Type: | Article | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Related URLs: |
| |||||||||
| ORCID: |
| |||||||||
| Additional Information: | © 1996 American Chemical Society. Received March 14, 1996. Publication Date (Web): July 17, 1996. We are grateful to the National Institutes of Health (Grant GM-35724) for research support and to the Howard Hughes Medical Institute for a predoctoral fellowship to E.E.B. | |||||||||
| Funders: |
| |||||||||
| Issue or Number: | 28 | |||||||||
| DOI: | 10.1021/ja960831y | |||||||||
| Record Number: | CaltechAUTHORS:20160510-102731520 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-102731520 | |||||||||
| Official Citation: | Cooperative Triple-Helix Formation via a Minor Groove Dimerization Domain Jason W. Szewczyk, Eldon E. Baird, and Peter B. Dervan Journal of the American Chemical Society 1996 118 (28), 6778-6779 DOI: 10.1021/ja960831y | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 66884 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Victoria Brennan | |||||||||
| Deposited On: | 18 May 2016 21:00 | |||||||||
| Last Modified: | 11 Nov 2021 00:23 |
Repository Staff Only: item control page
