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Cooperative triple-helix formation via a minor groove dimerization domain

Szewczyk, Jason W. and Baird, Eldon E. and Dervan, Peter B. (1996) Cooperative triple-helix formation via a minor groove dimerization domain. Journal of the American Chemical Society, 118 (28). pp. 6778-6779. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20160510-102731520

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Abstract

Cooperative interactions between DNA-binding proteins are critical to the regulation of gene expression. When cooperative interactions occur between two oligonucleotides bound at neighboring sites on double-helical DNA, the specific binding of each oligonucleotide is enhanced. Pyrimidine oligodeoxyribonucleotides 11 bases in length bind cooperatively to abutting sites on double-helical DNA by triple-helix formation. Cooperative interactions up to 2.3 kcal mol^(-1) were obtained by addition of dimerization domains to oligonucleotides such as those capable of forming a short Watson−Crick helix. The designed polyamide ImPyPy binds sequence specifically to the minor groove of DNA at 5‘-(T,A)G(T,A)C(T,A)-3‘ sites as a cooperative side-by-side antiparallel homodimer. We report here that a pyrrole−imidazole polyamide oligonucleotide can bind cooperatively to a double-stranded DNA template via a sequence specific polyamide dimerization domain.


Item Type:Article
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URLURL TypeDescription
http://dx.doi.org/10.1021/ja960831yDOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja960831yPublisherArticle
Additional Information:© 1996 American Chemical Society. Received March 14, 1996. Publication Date (Web): July 17, 1996. We are grateful to the National Institutes of Health (Grant GM-35724) for research support and to the Howard Hughes Medical Institute for a predoctoral fellowship to E.E.B.
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Funding AgencyGrant Number
NIHGM-35724
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Record Number:CaltechAUTHORS:20160510-102731520
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160510-102731520
Official Citation:Cooperative Triple-Helix Formation via a Minor Groove Dimerization Domain Jason W. Szewczyk, Eldon E. Baird, and Peter B. Dervan Journal of the American Chemical Society 1996 118 (28), 6778-6779 DOI: 10.1021/ja960831y
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66884
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:18 May 2016 21:00
Last Modified:18 May 2016 21:00

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