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Extended hairpin polyamide motif for sequence-specific recognition in the minor groove of DNA

Trauger, John W. and Baird, Eldon E. and Dervan, Peter B. (1996) Extended hairpin polyamide motif for sequence-specific recognition in the minor groove of DNA. Chemistry and Biology, 3 (5). pp. 369-377. ISSN 1074-5521. https://resolver.caltech.edu/CaltechAUTHORS:20160510-104009340

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Abstract

Three-ring polyamides containing N-methylimidazole and N-methylpyrrole amino acids bind sequence-specifically to double-helical DNA by forming side-by-side complexes in the minor groove. Simple pairing rules relate the amino-acid sequence of a pyrrole-imidazole polyamide to its expected DNA target site, and polyamides that target a wide variety of DNA sequences have been synthesized. We have shown previously that two three-ring subunits could be linked together by an aliphatic amino acid, increasing the binding affinity of the polyamide and, in some cases, increasing the length of the target sequence. We set out to determine whether different types of linkers could be used in a single molecule to generate a nine-ring polyamide molecule that would bind to specific DNA sequences.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S1074-5521(96)90120-9DOIArticle
http://www.sciencedirect.com/science/article/pii/S1074552196901209PublisherArticle
Additional Information:© 1996 Current Biology Ltd. Received 28 March 1996, Revised 26 April 1996, Accepted 26 April 1996. We are grateful to the National Institutes of Health (Grant GM-27681) for research support, to the National Science Foundation for a predoctoral fellowship to J.W.T., and to the Howard Hughes Medical Institute for a predoctoral fellowship to E.E.B.
Funders:
Funding AgencyGrant Number
NIHGM-27681
NSF Predoctoral FellowshipUNSPECIFIED
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Subject Keywords:binding affinity; linker amino acid; pyrrole-imidazole; solid-phase synthesis
Issue or Number:5
Record Number:CaltechAUTHORS:20160510-104009340
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-104009340
Official Citation:John W. Trauger, Eldon E. Baird, Peter B. Dervan, Extended hairpin polyamide motif for sequence-specific recognition in the minor groove of DNA, Chemistry & Biology, Volume 3, Issue 5, May 1996, Pages 369-377, ISSN 1074-5521, http://dx.doi.org/10.1016/S1074-5521(96)90120-9. (http://www.sciencedirect.com/science/article/pii/S1074552196901209)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66891
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:18 May 2016 21:27
Last Modified:03 Oct 2019 10:01

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