Parks, M. E. and Dervan, P. B. (1996) Simultaneous binding of a polyamide dimer and an oligonucleotide in the minor and major grooves of DNA. Bioorganic and Medicinal Chemistry, 4 (7). pp. 1045-50. ISSN 0968-0896. doi:10.1016/0968-0896(96)00089-2. https://resolver.caltech.edu/CaltechAUTHORS:20160510-104010083
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Abstract
The effect of the polyamide ImPyPy-Dp (Im = N-methylimidazole-2-carboxamide, Py = N-methylpyrrole-2-carboxamide, and Dp = dimethylaminopropylamide), which binds as an antiparallel dimer in the Watson—Crick minor groove, on pyrimidine • purine • pyrimidine triple helix stability was investigated. A DNA restriction fragment was designed which contained two triple helix sites, one which overlapped a minor groove ligand site (proximal), and a control site 13 base pairs away (distal). Using quantitative DNase I footprint titration experiments the equilibrium association constant of oligonucleotide 5′-TTTTTm5CTTTm5CTTTm5CT-3′ (1) to each site was measured in the absence and presence of the polyamide dimer. Our data indicate that triple helix formation is compatible with a polyamide dimer binding in the minor groove of DNA at an overlapping site. No cooperative effect of the polyamide dimer on the equilibrium association constant of oligonucleotide 1 was observed.
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Additional Information: | Copyright © 1996 Published by Elsevier Ltd. Received 20 March 1996, Accepted 21 March 1996, Available online 12 December 1998 We are grateful to the National Institutes of Health (GM-27681) for research support and a research service award to M.E.P. | |||||||||
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Issue or Number: | 7 | |||||||||
DOI: | 10.1016/0968-0896(96)00089-2 | |||||||||
Record Number: | CaltechAUTHORS:20160510-104010083 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-104010083 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 66892 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Victoria Brennan | |||||||||
Deposited On: | 18 May 2016 21:34 | |||||||||
Last Modified: | 11 Nov 2021 00:23 |
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