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Energetics of Formation of Sixteen Triple Helical Complexes Which Vary at a Single Position Within a Purine Motif

Greenberg, William A. and Dervan, Peter B. (1995) Energetics of Formation of Sixteen Triple Helical Complexes Which Vary at a Single Position Within a Purine Motif. Journal of the American Chemical Society, 117 (18). pp. 5016-5022. ISSN 0002-7863. doi:10.1021/ja00123a002. https://resolver.caltech.edu/CaltechAUTHORS:20160510-104434541

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Abstract

The association constants for the formation of sixteen purine purine pyrimidine triple helical complexes which vary at a single common position (Z . XY where Z = A, G, C, T and XY = AT, GC, CG, TA) were determined by quantitative DNAse I footprint titration. The sequence context in which the sixteen pairings Z . XY were studied is 5'-d(AGGGGAGGGGXGGGA)-3' within a 648 base pair DNA fragment. The association constants for the purine-rich oligonucleotides 5'-d(TGGGZGGGGTGGGGT)-3' ranged from 2.7 x 10^5 (Z . XY = G . TA) to 8.9 x 10^7 M^(-1) (Z . XY = T . AT) at 37 degrees C, pH 7,4, 50 mM tris acetate, 10 mM NaCl, and 3 mM MgCl_2. For the sequences studied, T . AT is more stable than T . CG, T . GC, and T . TA by greater than or equal to 1.2 kcal mol^(-1); A . AT is more stable than A . CG, A . GC, and A . TA by greater than or equal to 1.2 kcal mol^(-1); and G . GC is more stable than G . AT, G . CG, and G . TA by greater than or equal to 2.1 kcal mol^(-1). The triplet T . CG is greater than or equal to 1.5 kcal mol^(-1) more stable than the other three bases opposite a CG base pair.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja00123a002DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00123a002PublisherArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1995 American Chemical Society. Received December 12, 1994. Publication Date: May 1995. Abstract published in Advance ACS Abstracts, April 15, 1995. We thank Dr. Peter Bed for the plasmids used in this study and for helpful discussions. We are grateful for generous support from the National Institutes of Health (GM35724) and the Office of Naval Research and for a National Science Foundation predoctoral fellowship to W.A.G.
Funders:
Funding AgencyGrant Number
NIHGM-35724
Office of Naval Research (ONR)UNSPECIFIED
NSF Predoctoral FellowshipUNSPECIFIED
Issue or Number:18
DOI:10.1021/ja00123a002
Record Number:CaltechAUTHORS:20160510-104434541
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-104434541
Official Citation:Energetics of Formation of Sixteen Triple Helical Complexes Which Vary at a Single Position Within a Purine Motif William A. Greenberg and Peter B. Dervan Journal of the American Chemical Society 1995 117 (18), 5016-5022 DOI: 10.1021/ja00123a002
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66893
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:18 May 2016 22:16
Last Modified:11 Nov 2021 00:23

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