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Temperature Dependence of the Energetics of Oligonucleotide-Directed Triple-Helix Formation at a Single DNA Site

Singleton, Scott F. and Dervan, Peter B. (1994) Temperature Dependence of the Energetics of Oligonucleotide-Directed Triple-Helix Formation at a Single DNA Site. Journal of the American Chemical Society, 116 (23). pp. 10376-10382. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20160510-110239191

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Abstract

The influence of temperature on the energetics of oligonucleotide-directed triple-helix formation has been investigated in mixed valence salt solutions at pH 7.0. Equilibrium constants for formation of the local pyrimidine.purine.pyrimidine structure afforded by binding of the oligonucleotide 5'-d(T*TTTTCTCTCTCTCT)-3' to a single 15-bp site within a 339-bp plasmid fragment were measured using quantitative affinity cleavage titrations between 8 and 37 °C. In three different solutions buffered by 10 mM Bis-Tris at pH 7.0, BTNS 100 mM NaCl and 1 mM spermine tetrahydrochloride (SpmCl_4)], BTP1 (10 mM NaCl, 140 mM KCl, 1 mM MgCl_2, and 1 mM SpmCl_4), and BTP4 (10 mM NaCl, 140 mM KCl, 1 mM MgCl_2, and 4 mM SpmCl_4), the equilibrium association constants decreased at least 100-fold (from > 10^7 M^(-1) to ca. 10^5 M^(-1)) as the temperature was increased from 8 to 37 °C. Least squares analysis of van't Hoff plots (In K versus 1/T) of the data revealed that in each solution the tripler is enthalpically stabilized by ca. 2 kcal per mol of base triplets. This average value for single-site tripler formation on large DNA at pH 7.0 in mixed valence salt solutions, which reflects contributions from both T•AT and C+GC base triplets, is consistent with those reported previously for a number of oligonucleotide triplexes in solutions containing single cationic species and in excellent agreement with the calorimetrically determined enthalpy for binding of this 15mer to a 21-bp oligonucleotide DNA duplex.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja00102a003DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00102a003PublisherArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1994 American Chemical Society. Received May 27, 1994. Publication Date: November 1994. Abstract published in Advance ACS Abstracts, November 1, 1994. We are grateful to the National Institutes of Health for generous research support (GM-35724) and a National Research Service Award to S.F.S. and to the Ralph M. Parsons Foundation for a predoctoral fellowship to S.F.S.
Funders:
Funding AgencyGrant Number
NIHGM-35724
NIH Predoctoral FellowshipUNSPECIFIED
Ralph M. Parsons FoundationUNSPECIFIED
Issue or Number:23
Record Number:CaltechAUTHORS:20160510-110239191
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-110239191
Official Citation:Temperature Dependence of the Energetics of Oligonucleotide-Directed Triple-Helix Formation at a Single DNA Site Scott F. Singleton and Peter B. Dervan Journal of the American Chemical Society 1994 116 (23), 10376-10382 DOI: 10.1021/ja00102a003
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66900
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:18 May 2016 23:26
Last Modified:26 Nov 2019 11:15

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