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Only one of the two DNA-bound orientations of AP-1 found in solution cooperates with NFATp

Chen, Lin and Oakley, Martha G. and Glover, J. N. Mark and Jain, Jugnu and Dervan, Peter B. and Hogan, Patrick G. and Rao, Anjana and Verdine, Gregory L. (1995) Only one of the two DNA-bound orientations of AP-1 found in solution cooperates with NFATp. Current Biology, 5 (8). pp. 882-889. ISSN 0960-9822. https://resolver.caltech.edu/CaltechAUTHORS:20160510-110522381

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Abstract

The transcription factor AP-1 activates the expression of numerous genes in response to mitogenic stimuli. AP-1 regulates gene expression both through solitary binding to independent recognition sites and, in cooperation with various heterologous transcription factors, through targeting to composite response elements. The two subunits that make up the AP-1 heterodimer, Fos and Jun, possess identical residues at positions that make sequence-specific contacts to DNA. This degeneracy leaves the protein with no apparent way of orienting itself uniquely on DNA by differentially recognizing its two non-identical half-sites. Here, we have analyzed the orientation of the AP-1 basic-leucine-zipper (bZip) domain on a cognate site, both alone and in the cooperative complex formed together with the 'nuclear factor of activated T cells' (NFATp).


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0960-9822(95)00178-3DOIArticle
http://www.sciencedirect.com/science/article/pii/S0960982295001783PublisherArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1995 Elsevier Science Ltd. Received 27 March 1995, Revised 5 June 1995, Accepted 6 June 1995. This work was supported by grants (to G.L.V. and A.R.) from Hoffmann-La Roche (Institute of Chemistry and Medicine) and (to P.B.D.) National Institutes of Health (GM-27681). M.G.O was partially supported by a predoctoral fellowship from the National Science Foundation. We thank Steve Harrison for discussions and for sharing the coordinates of the Fos-Jun co-complex prior to publication.
Funders:
Funding AgencyGrant Number
NIHGM-27681
Hoffmann-La Roche FoundationUNSPECIFIED
NSF Predoctoral FellowshipUNSPECIFIED
Issue or Number:8
Record Number:CaltechAUTHORS:20160510-110522381
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-110522381
Official Citation:Lin Chen, Martha G. Oakley, J.N. Mark Glover, Jugnu Jain, Peter B. Dervan, Patrick G. Hogan, Anjana Rao, Gregory L. Verdine, Only one of the two DNA-bound orientations of AP-1 found in solution cooperates with NFATp, Current Biology, Volume 5, Issue 8, August 1995, Pages 882-889, ISSN 0960-9822, http://dx.doi.org/10.1016/S0960-9822(95)00178-3. (http://www.sciencedirect.com/science/article/pii/S0960982295001783)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66904
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:18 May 2016 22:03
Last Modified:26 Nov 2019 11:15

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