Staubli, Andrea B. and Dervan, Peter B. (1994) Sequence specificity of the non-natural pyrido[2,3-d]pyrimidine nucleoside in triple helix formation. Nucleic Acids Research, 22 (13). pp. 2637-2642. ISSN 0305-1048. PMCID PMC308221. https://resolver.caltech.edu/CaltechAUTHORS:20160510-132907922
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Abstract
The non-natural pyrido[2,3-d]pyrimidine nucleoside F, which pairs preferentially with guanine (G) and adenine (A) within double-helical DNA, recognizes with high selectivity AT base pairs within triple-helical complexes. These observations suggest that F may exist in different tautomeric forms within double-helical and triple-helical complexes. Analysis of the base stacking properties of this extended ring system using two oligodeoxyribonucleotides containing terminal thymines and/or pyrido[2,3-d]pyrimidines bound to adjacent sites showed a decrease in free energy of binding in a triple-helical complex in the order (5′ – 3′) TT > FT > TF > FF.
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Additional Information: | © 1994 Oxford University Press. Received March 4, 1994; Revised and Accepted May 17, 1994. Plasmids pPG1133 and pMD5152 were obtained from Justine Garvey. We are thankful for the support from the Office of Naval Research and for a postdoctoral fellowship from the Swiss National Science Foundation to A.B.S. | ||||||||||||
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Issue or Number: | 13 | ||||||||||||
PubMed Central ID: | PMC308221 | ||||||||||||
Record Number: | CaltechAUTHORS:20160510-132907922 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-132907922 | ||||||||||||
Official Citation: | Andrea B. Staubli and Peter B. Dervan Sequence specificity of the non-natural pyrido[2,3-d]pyrimidine nucleoside in triple helix formation Nucl. Acids Res. (1994) 22 (13): 2637-2642 doi:10.1093/nar/22.13.2637 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 66910 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | Victoria Brennan | ||||||||||||
Deposited On: | 19 May 2016 16:43 | ||||||||||||
Last Modified: | 26 Nov 2019 11:15 |
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