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Sequence specificity of the non-natural pyrido[2,3-d]pyrimidine nucleoside in triple helix formation

Staubli, Andrea B. and Dervan, Peter B. (1994) Sequence specificity of the non-natural pyrido[2,3-d]pyrimidine nucleoside in triple helix formation. Nucleic Acids Research, 22 (13). pp. 2637-2642. ISSN 0305-1048. PMCID PMC308221. https://resolver.caltech.edu/CaltechAUTHORS:20160510-132907922

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Abstract

The non-natural pyrido[2,3-d]pyrimidine nucleoside F, which pairs preferentially with guanine (G) and adenine (A) within double-helical DNA, recognizes with high selectivity AT base pairs within triple-helical complexes. These observations suggest that F may exist in different tautomeric forms within double-helical and triple-helical complexes. Analysis of the base stacking properties of this extended ring system using two oligodeoxyribonucleotides containing terminal thymines and/or pyrido[2,3-d]pyrimidines bound to adjacent sites showed a decrease in free energy of binding in a triple-helical complex in the order (5′ – 3′) TT > FT > TF > FF.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1093/nar/22.13.2637DOIArticle
http://nar.oxfordjournals.org/content/22/13/2637PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC308221/PubMed CentralArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1994 Oxford University Press. Received March 4, 1994; Revised and Accepted May 17, 1994. Plasmids pPG1133 and pMD5152 were obtained from Justine Garvey. We are thankful for the support from the Office of Naval Research and for a postdoctoral fellowship from the Swiss National Science Foundation to A.B.S.
Funders:
Funding AgencyGrant Number
Office of Naval Research (ONR)UNSPECIFIED
Swiss National Science Foundation (SNSF)UNSPECIFIED
Issue or Number:13
PubMed Central ID:PMC308221
Record Number:CaltechAUTHORS:20160510-132907922
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-132907922
Official Citation:Andrea B. Staubli and Peter B. Dervan Sequence specificity of the non-natural pyrido[2,3-d]pyrimidine nucleoside in triple helix formation Nucl. Acids Res. (1994) 22 (13): 2637-2642 doi:10.1093/nar/22.13.2637
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66910
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:19 May 2016 16:43
Last Modified:26 Nov 2019 11:15

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