Sequence specificity of the non-natural pyrido[2,3-d]pyrimidine nucleoside in triple helix formation

Staubli, Andrea B. and Dervan, Peter B. (1994) Sequence specificity of the non-natural pyrido[2,3-d]pyrimidine nucleoside in triple helix formation. Nucleic Acids Research, 22 (13). pp. 2637-2642. ISSN 0305-1048. PMCID PMC308221. https://resolver.caltech.edu/CaltechAUTHORS:20160510-132907922

PDF - Published Version
Creative Commons Attribution.
3152Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20160510-132907922

Abstract

The non-natural pyrido[2,3-d]pyrimidine nucleoside F, which pairs preferentially with guanine (G) and adenine (A) within double-helical DNA, recognizes with high selectivity AT base pairs within triple-helical complexes. These observations suggest that F may exist in different tautomeric forms within double-helical and triple-helical complexes. Analysis of the base stacking properties of this extended ring system using two oligodeoxyribonucleotides containing terminal thymines and/or pyrido[2,3-d]pyrimidines bound to adjacent sites showed a decrease in free energy of binding in a triple-helical complex in the order (5′ – 3′) TT > FT > TF > FF.

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1093/nar/22.13.2637	DOI	Article
http://nar.oxfordjournals.org/content/22/13/2637	Publisher	Article
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC308221/	PubMed Central	Article

ORCID:

Author	ORCID
Dervan, Peter B.	0000-0001-8852-7306

Additional Information:

© 1994 Oxford University Press. Received March 4, 1994; Revised and Accepted May 17, 1994. Plasmids pPG1133 and pMD5152 were obtained from Justine Garvey. We are thankful for the support from the Office of Naval Research and for a postdoctoral fellowship from the Swiss National Science Foundation to A.B.S.

Funders:

Funding Agency	Grant Number
Office of Naval Research (ONR)	UNSPECIFIED
Swiss National Science Foundation (SNSF)	UNSPECIFIED

Issue or Number:

PubMed Central ID:

PMC308221

Record Number:

CaltechAUTHORS:20160510-132907922

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20160510-132907922

Official Citation:

Andrea B. Staubli and Peter B. Dervan Sequence specificity of the non-natural pyrido[2,3-d]pyrimidine nucleoside in triple helix formation Nucl. Acids Res. (1994) 22 (13): 2637-2642 doi:10.1093/nar/22.13.2637

Usage Policy:

No commercial reproduction, distribution, display or performance rights in this work are provided.

ID Code:

66910

Collection:

CaltechAUTHORS

Deposited By:

Victoria Brennan

Deposited On:

19 May 2016 16:43

Last Modified:

26 Nov 2019 11:15

Repository Staff Only: item control page