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Cooperative Binding of 8-mer Oligonucleotides Containing 5-(1-Propynyl)-2'-deoxyuridine to Adjacent DNA Sites by Triple-Helix Formation

Colocci, Natalia and Dervan, Peter B. (1994) Cooperative Binding of 8-mer Oligonucleotides Containing 5-(1-Propynyl)-2'-deoxyuridine to Adjacent DNA Sites by Triple-Helix Formation. Journal of the American Chemical Society, 116 (2). pp. 785-786. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20160510-133354203

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Abstract

The design of cooperative domains between DNA-binding ligands for modulation of the kinetics and thermodynamics of ligand- nucleic acid interactions is at an early stage of development. Pyrimidine oligodeoxyribonucleotides 11 nucleotides (nt) in length are known to bind cooperatively to abutting sites on double-helical DNA by triple-helix formation, resulting in a 20-fold increase in the association constant for one oligonucleotide in the presence of the neighboring oligonucleotide. This 1.8 kcal·mol^(-1) interaction energy may arise from π-stacking between the bases at the triple-helical junction. The incorporation of discrete dimerization domains to oligonucleotides such as those capable of forming a short Watson-Crick helix produces a cooperative interaction and results in a 44-fold enhancement in the association constant of one oligonucleotide in the presence of a neighbor.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja00081a056DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00081a056PublisherArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1994 American Chemical Society. Received October 18, 1993. We are grateful to the National Institutes of Health for grant support (HG 00329) and for a National Research Service Award (Predoctoral Training in Genome Analysis HG00021-03) to N.C.
Funders:
Funding AgencyGrant Number
NIHHG00329
NIH Predoctoral FellowshipHG00021-03
Issue or Number:2
Record Number:CaltechAUTHORS:20160510-133354203
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-133354203
Official Citation:Cooperative Binding of 8-mer Oligonucleotides Containing 5-(1-Propynyl)-2'-deoxyuridine to Adjacent DNA Sites by Triple-Helix Formation Natalia Colocci and Peter B. Dervan Journal of the American Chemical Society 1994 116 (2), 785-786 DOI: 10.1021/ja00081a056
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66912
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:19 May 2016 16:49
Last Modified:26 Nov 2019 11:15

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