Colocci, Natalia and Dervan, Peter B. (1994) Cooperative Binding of 8-mer Oligonucleotides Containing 5-(1-Propynyl)-2'-deoxyuridine to Adjacent DNA Sites by Triple-Helix Formation. Journal of the American Chemical Society, 116 (2). pp. 785-786. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20160510-133354203
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Abstract
The design of cooperative domains between DNA-binding ligands for modulation of the kinetics and thermodynamics of ligand- nucleic acid interactions is at an early stage of development. Pyrimidine oligodeoxyribonucleotides 11 nucleotides (nt) in length are known to bind cooperatively to abutting sites on double-helical DNA by triple-helix formation, resulting in a 20-fold increase in the association constant for one oligonucleotide in the presence of the neighboring oligonucleotide. This 1.8 kcal·mol^(-1) interaction energy may arise from π-stacking between the bases at the triple-helical junction. The incorporation of discrete dimerization domains to oligonucleotides such as those capable of forming a short Watson-Crick helix produces a cooperative interaction and results in a 44-fold enhancement in the association constant of one oligonucleotide in the presence of a neighbor.
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| Additional Information: | © 1994 American Chemical Society. Received October 18, 1993. We are grateful to the National Institutes of Health for grant support (HG 00329) and for a National Research Service Award (Predoctoral Training in Genome Analysis HG00021-03) to N.C. | |||||||||
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| Issue or Number: | 2 | |||||||||
| Record Number: | CaltechAUTHORS:20160510-133354203 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-133354203 | |||||||||
| Official Citation: | Cooperative Binding of 8-mer Oligonucleotides Containing 5-(1-Propynyl)-2'-deoxyuridine to Adjacent DNA Sites by Triple-Helix Formation Natalia Colocci and Peter B. Dervan Journal of the American Chemical Society 1994 116 (2), 785-786 DOI: 10.1021/ja00081a056 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 66912 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Victoria Brennan | |||||||||
| Deposited On: | 19 May 2016 16:49 | |||||||||
| Last Modified: | 26 Nov 2019 11:15 |
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