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Mapping RNA Regions in Eukaryotic Ribosomes That Are Accessible to Methidiumpropyl-EDTA•Fe(II) and EDTA•Fe(II)

Han, Hogyu and Schepartz, Alanna and Pellegrini, Maria and Dervan, Peter B. (1994) Mapping RNA Regions in Eukaryotic Ribosomes That Are Accessible to Methidiumpropyl-EDTA•Fe(II) and EDTA•Fe(II). Biochemistry, 33 (33). pp. 9831-9844. ISSN 0006-2960. https://resolver.caltech.edu/CaltechAUTHORS:20160510-133744054

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Abstract

Methidiumpropyl-EDTA•Fe(II) [MPE.Fe(II)] and EDTA•Fe(II) were used to investigate the structure of Drosophila melanogaster ribosomes. Cleavage reactions were performed on intact ribosomes in cell lysates in vitro and analyzed by primer extension with reverse transcriptase using oligodeoxynucleotide primers. Regions of 18S and 28S ribosomal RNAs (rRNAs) which are accessible to MPE•Fe(II) and EDTA•Fe(II) are located almost exclusively within expansion segments. The accessibility of these regions to cleavage indicates that they are likely exposed on the surface of eukaryotic ribosomes. These results provide information about the overall tertiary structure of rRNA in ribosomes.


Item Type:Article
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1994 American Chemical Society. Received April 4, 1994; Revised Manuscript Received May 31, 1994. Abstract published in Advance ACS Abstracts, August 1, 1994. This research was supported by National Institutes of Health grants [GM-27681 (P.B.D.) and GM-45299, 40857 (M.P.)] and by an NIH postdoctoral fellowship (GM-11646-02) to AS. We thank Drs. Lorna Neilson, Shawn Vallett, and Heather W. Weber for helpful discussions. We are grateful to Dr. Robin R. Gutell for providing secondary structural models.
Funders:
Funding AgencyGrant Number
NIHGM-27681
NIHGM-45299
NIHGM-40857
NIH Postdoctoral FellowshipGM-11646-02
Issue or Number:33
Record Number:CaltechAUTHORS:20160510-133744054
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-133744054
Official Citation:Mapping RNA Regions in Eukaryotic Ribosomes That Are Accessible to Methidiumpropyl-EDTA•Fe(II) and EDTA•Fe(II) Hogyu Han, Alanna Schepartz, Maria Pellegrini, and Peter B. Dervan Biochemistry 1994 33 (33), 9831-9844 DOI: 10.1021/bi00199a004
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66915
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:18 May 2016 22:12
Last Modified:26 Nov 2019 11:15

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