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Different conformational families of pyrimidine·purine·pyrimidine triple helices depending on backbone composition

Han, Hogyu and Dervan, Peter B. (1994) Different conformational families of pyrimidine·purine·pyrimidine triple helices depending on backbone composition. Nucleic Acids Research, 22 (14). pp. 2837-2844. ISSN 0305-1048. PMCID PMC308255. https://resolver.caltech.edu/CaltechAUTHORS:20160510-133745163

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Abstract

Different helical conformations of DNA (D), RNA (R), and DNA•RNA (DR) hybrid double and triple helices have been detected using affinity cleavage analysis. Synthetic methods were developed to attach EDTA•Fe to a single nucleotide on RNA as well as DNA oligonucleotides. Cleavage patterns generated by a localized diffusible oxidant in the major groove on the pyrimidine strand of four purine.pyrimidine double helices consisting of all DNA, all RNA, and the corresponding hybrids reveal that the relative cleavage intensity shifts to the 5' end of the purine strand increasingly in the order: DD < DR < RD < RR. These results are consistent with models derived from structural studies. In six pyrimidine.purine.pyrimidine triple helices, the altered cleavage patterns of the Watson-Crick pyrimidine strands reveal at least two conformational families: (i) D + DD, R + DD, D + DR, and R + DR and (ii) R + RD and R + RR.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1093/nar/22.14.2837DOIArticle
http://nar.oxfordjournals.org/content/22/14/2837.abstractPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC308255/PubMed CentralArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1994 Oxford University Press. Received January 18, 1994. Revision received May 12, 1994. Accepted May 12, 1994. We are grateful to the National Institutes of Health for grant support.
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NIHUNSPECIFIED
Issue or Number:14
PubMed Central ID:PMC308255
Record Number:CaltechAUTHORS:20160510-133745163
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-133745163
Official Citation:Hogyu Han and Peter B. Dervan Different conformational families of pyrimidine·purine·pyrimidine triple helices depending on backbone composition Nucl. Acids Res. (1994) 22 (14): 2837-2844 doi:10.1093/nar/22.14.2837
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66916
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:18 May 2016 23:04
Last Modified:26 Nov 2019 11:15

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