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Phosphorothioate oligonucleotide-directed triple helix formation

Hacia, Joseph G. and Wold, Barbara J. and Dervan, Peter B. (1994) Phosphorothioate oligonucleotide-directed triple helix formation. Biochemistry, 33 (18). pp. 5367-5369. ISSN 0006-2960.

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Phosphorothioate oligodeoxyribonucleotides were tested for their ability to recognize double-helical DNA in two distinct triple helix motifs. Purine-rich oligonucleotides containing a diastereomeric mixture of phosphorothioate or stereoregular (all RP) phosphorothioate linkages are shown to form triple-helical complexes with affinities similar to those of the corresponding natural phosphodiester oligonucleotides. In contrast, pyrimidine-rich phosphorothioate oligonucleotides containing a mixture of diastereomeric or stereoregular (all RP) linkages do not bind to double-helical DNA with measurable affinity. These observations have implications for triple helix structure and for biological applications.

Item Type:Article
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URLURL TypeDescription
Wold, Barbara J.0000-0003-3235-8130
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1994 American Chemical Society. Received February 4, 1994; Revised Manuscript Received March 7, 1994. Abstract published in Advance ACS Abstracts, April 15, 1994. Supported by a predoctoral fellowship from the Howard Hughes Medical Institute (to J.G.H.) and by research grants from the NIH (AR40780 to B.J.W. and GM35724 to P.B.D.).
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Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Issue or Number:18
Record Number:CaltechAUTHORS:20160510-133746868
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Official Citation:Phosphorothioate Oligonucleotide-Directed Triple Helix Formation Joseph G. Hacia, Barbara J. Wold, and Peter B. Dervan Biochemistry 1994 33 (18), 5367-5369 DOI: 10.1021/bi00184a002
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66918
Deposited By: Victoria Brennan
Deposited On:18 May 2016 23:16
Last Modified:09 Mar 2020 13:19

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