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Structural and dynamic characterization of the heterodimeric and homodimeric complexes of distamycin and 1-methylimidazole-2-carboxamide-netropsin bound to the minor groove of DNA

Geierstanger, Bernhard H. and Jacobsen, Jens Peter and Mrksich, Milan and Dervan, Peter B. and Wemmer, David E. (1994) Structural and dynamic characterization of the heterodimeric and homodimeric complexes of distamycin and 1-methylimidazole-2-carboxamide-netropsin bound to the minor groove of DNA. Biochemistry, 33 (10). pp. 3055-3062. ISSN 0006-2960. https://resolver.caltech.edu/CaltechAUTHORS:20160510-133748441

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Abstract

NMR spectroscopy combined with molecular modeling was used to characterize a heterodimeric complex with Dst and 2-ImN bound in the minor groove of d(GCCTAACAAGG)•d(CCTTGTTAGGC) (1:1:1 2-ImN•Dst•DNA complex). The imidazole-pyrrole-pyrrole ligand 2-ImN spans 5'-GTTA-3' of the TAACA•TGTTA binding site with the imidazole nitrogen specifically recognizing the guanine amino group. The Dst ligand lies along the 5'-AACA-3' sequence and complements the 2-ImN ligand in the formation of the antiparallel side-by-side heterodimeric complex. Titrations of the same site with Dst or 2-ImN alone yield homodimeric complexes (2:1 ligand.DNA) of lower stability than the 1:1:1 2-ImN•Dst•DNA complex. Dst and 2-ImN binding to d(CGCAAACTGGC)•d(GCCAGTTTGCG) was also investigated. The 1:1:1 2-ImN•Dst•DNA complex is again the most stable complex with the AAACT•AGTTT site and is similar to the TAACA•TGTTA complex. No monomeric binding of either 2-ImN or Dst was observed to either site.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi00176a039DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi00176a039PublisherArticle
http://pubs.acs.org/doi/suppl/10.1021/bi00176a039PublisherSupporting Information
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1994 American Chemical Society. Received October 20, 1993; Revised Manuscript Received December 14, 1993. Abstract published in Advance ACS Abstracts, February 15, 1994. We are grateful to the National Institutes of Health (GM-27681 to P.B.D. and GM-43129 to D.E.W.) and the National Foundation for Cancer Research for research support and for a National Institutes of Health Research Service Award to M.M. and to the US. Department of Energy (DE FG05-86ER75281) and the National Science Foundation (DMB 86-09305 and BBS 87-20134) for instrumentation grants.
Funders:
Funding AgencyGrant Number
NIHGM-27681
NIHGM-43129
National Foundation for Cancer ResearchUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Department of Energy (DOE)DE FG05-86ER75281
NSFDMB 86-09305
NSFBBS 87-20134
Issue or Number:10
Record Number:CaltechAUTHORS:20160510-133748441
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-133748441
Official Citation:Structural and Dynamic Characterization of the Heterodimeric and Homodimeric Complexes of Distamycin and 1-Methylimidazole-2-carboxamide-netropsin Bound to the Minor Groove of DNA Bernhard H. Geierstanger, Jens Peter Jacobsen, Milan Mrksich, Peter B. Dervan, and David E. Wemmer Biochemistry 1994 33 (10), 3055-3062 DOI: 10.1021/bi00176a039
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66920
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:18 May 2016 23:24
Last Modified:26 Nov 2019 11:15

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