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Crystallization and preliminary X-ray analysis of the DNA binding domain of the Hin recombinase with its DNA binding site

Feng, Jin-An and Simon, Melvin and Mack, David P. and Dervan, Peter B. and Johnson, Reid C. and Dickerson, Richard E. (1993) Crystallization and preliminary X-ray analysis of the DNA binding domain of the Hin recombinase with its DNA binding site. Journal of Molecular Biology, 232 (3). pp. 982-986. ISSN 0022-2836. https://resolver.caltech.edu/CaltechAUTHORS:20160510-140054373

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Abstract

The Hin recombinase catalyzes site-specific inversion of DNA. Chemical and genetic studies on Hin binding to the recombination sites indicates that both major and minor DNA groove interactions are critical. In order to determine the molecular nature of these interactions, we have crystallized a synthetically derived 52 amino acid peptide consisting of the DNA binding domain of Hin with a 14 base-pair oligonucleotide representing a recombination half-site. This communication presents preliminary diffraction and analysis of these cocrystals and a packing model for the complex within the crystal.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1006/jmbi.1993.1443DOIArticle
http://www.sciencedirect.com/science/article/pii/S0022283683714439PublisherArticle
Additional Information:© 1993 Academic Press. Received 26 October 1992; accepted 16 March 1993. We thank Dohn Glitz for advice and help in DNA synthesis, and Steven Finkel for help in DNA synthesis and purification. We also thank Duilio Cascio for assistance in data collection. This work was carried out with the support of research grants NIH GM-31299 to R.E.D., GM-38509 to R.C.J. and NSF grant DMB-8516021 to M.S.
Funders:
Funding AgencyGrant Number
NIHGM-31299
NIHGM-38509
NSFDMB-8516021
Subject Keywords:Hin recombinase; invertasome; protein/DNA complex; DNA-binding protein
Issue or Number:3
Record Number:CaltechAUTHORS:20160510-140054373
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-140054373
Official Citation:Jin-An Feng, Melvin Simon, David P. Mack, Peter B. Dervan, Reid C. Johnson, Richard E. Dickerson, Crystallization and Preliminary X-ray Analysis of the DNA Binding Domain of the Hin Recombinase with Its DNA Binding Site, Journal of Molecular Biology, Volume 232, Issue 3, 1993, Pages 982-986, ISSN 0022-2836, http://dx.doi.org/10.1006/jmbi.1993.1443. (http://www.sciencedirect.com/science/article/pii/S0022283683714439)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66931
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:19 May 2016 23:26
Last Modified:03 Oct 2019 10:01

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