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Ligand-promoted dimerization of oligonucleotides binding cooperatively to DNA

Distefano, Mark D. and Dervan, Peter B. (1992) Ligand-promoted dimerization of oligonucleotides binding cooperatively to DNA. Journal of the American Chemical Society, 114 (27). pp. 11006-11007. ISSN 0002-7863. doi:10.1021/ja00053a073.

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In biological systems, specific protein-DNA interactions are often modulated by ligands as in the regulation of gene expression. Many transcription factors arc active as dimers, but inactive as monomes. In at least one case, dimerization is regulated by an additional protein cofactor. We recently described the design of a triple helical complex compnsmg two oligonucleotides which bind adjacent sites on DNA cooperatively through dimerization. If dimerizat1on could be controlled by additional ligands such an artificial nucleic acid system would be analogous to the naturally occurring inducible protein systems. We now report that the stability of the dimerization domain can be enhanced by a small molecule, echinomycin (E). The strengths of ligand-promoted control of oligonucleotides binding cooperatively to DNA are quantitated by affinity cleaving.

Item Type:Article
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Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1992 American Chemical Society. Received September 18, 1992. Publication Date: December 1992. This work was supported by the National Institutes of Health (GM-35724) and a Damon Runyon- Walter Winchell Cancer Research Fund postdoctoral fellowship for M.D.D.
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Damon Runyon-Walter Winchell Cancer Research FundUNSPECIFIED
Issue or Number:27
Record Number:CaltechAUTHORS:20160510-140302676
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Official Citation:Ligand-promoted dimerization of oligonucleotides binding cooperatively to DNA Mark D. Distefano and Peter B. Dervan Journal of the American Chemical Society 1992 114 (27), 11006-11007 DOI: 10.1021/ja00053a073
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66933
Deposited By: Victoria Brennan
Deposited On:19 May 2016 17:06
Last Modified:11 Nov 2021 00:24

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