Wade, Warren S. and Mrksich, Milan and Dervan, Peter B. (1992) Design of peptides that bind in the minor groove of DNA at 5'-(A,T)G(A,T)C(A,T)-3' sequences by a dimeric side-by-side motif. Journal of the American Chemical Society, 114 (23). pp. 8783-8794. ISSN 0002-7863. doi:10.1021/ja00049a006. https://resolver.caltech.edu/CaltechAUTHORS:20160510-140855381
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Abstract
The designed peptides pyridine-2-carboxamide-netropsin (2-PyN) and 1-methylimidazole-2-carboxamide-netropsin (2-ImN) are crescent-shaped synthetic analogs of the natural products netropsin (N) and distamycin A (D). Footprinting experiments indicate that the peptides 2-PyN and 2-ImN bind specifically the 5 base pair sequence 5'-TGTCA-3'. Affinity cleaving data suggest that the complexes, 2-ImN.5'-TGTCA-3' and 2-PyN.5'-TGTCA-3', are composed of two equivalent orientations which disfavor a 1:1 model. The footprinting and affinity cleaving data are in accord with a 2:1 complex where a novel side-by-side antiparallel dimer binds in the minor groove of double-helical DNA.
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| Additional Information: | © 1992 American Chemical Society. Received May 11, 1992. Publication Date: November 1992. We are grateful to the National Institutes of Health (GM-27681) and to Burroughs Wellcome for research support, to the National Science Foundation for a predoctoral fellowship to W.S.W., and to the National Institutes of Health for a Research Service Award to M.M. | ||||||||||
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| Issue or Number: | 23 | ||||||||||
| DOI: | 10.1021/ja00049a006 | ||||||||||
| Record Number: | CaltechAUTHORS:20160510-140855381 | ||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-140855381 | ||||||||||
| Official Citation: | Design of peptides that bind in the minor groove of DNA at 5'-(A,T)G(A,T)C(A,T)-3' sequences by a dimeric side-by-side motif Warren S. Wade, Milan Mrksich, and Peter B. Dervan Journal of the American Chemical Society 1992 114 (23), 8783-8794 DOI: 10.1021/ja00049a006 | ||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||
| ID Code: | 66934 | ||||||||||
| Collection: | CaltechAUTHORS | ||||||||||
| Deposited By: | Victoria Brennan | ||||||||||
| Deposited On: | 19 May 2016 17:08 | ||||||||||
| Last Modified: | 11 Nov 2021 00:24 |
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