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Nonenymatic ligation of double-helical DNA by alternate-strand triple helix formation

Luebke, Kevin J. and Dervan, Peter B. (1992) Nonenymatic ligation of double-helical DNA by alternate-strand triple helix formation. Nucleic Acids Research, 20 (12). pp. 3005-3009. ISSN 0305-1048. PMCID PMC312430. doi:10.1093/nar/20.12.3005.

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Nonenzymatic ligation of double-stranded DNA has been performed using an alternate-strand binding oligodeoxyribonucleotide template to juxtapose the duplex termini in a triple helical complex. The template associates with the duplex termini by Hoogsteen hydrogen bonding to alternate strands on opposite sides of the ligation site. Intermolecular and intramolecular ligation of linearized plasmid DNA are observed in the reaction, which depends on the template oligodeoxyribonucleotide and a condensing agent, N-cyanoimidazole. Intramolecular ligation products include those in which both strands are covalently closed in a circle. Ligation of the two strands is sequential and occurs at comparable rates for the first and second strands ligating. The covalent linkages formed in the reaction can be cleaved by the restriction endonuclease Stu I, supporting their identification as phosphodiesters.

Item Type:Article
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Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1992 Oxford University Press. Received April 1, 1992. Revision received May 15, 1992. Accepted May 15, 1992. This work was supported in part by the National Institutes of Health (GM-35724) and the Caltech Consortium in Chemistry and Chemical Engineering (founding members: E.I.du Pont de Nemours and Company, Inc., Eastman Kodak Company, Minnesota Mining and Manufacturing Company, and Shell Oil Company Foundation). We are grateful for a National Research Service Award to K.J.L. from the National Institute of General Medical Science. 5-dimethoxytrityl-1,2-dideoxy-D-ribose was a gift of Thomas J. Povsic.
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Caltech Consortium in Chemistry and Chemical EngineeringUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Issue or Number:12
PubMed Central ID:PMC312430
Record Number:CaltechAUTHORS:20160510-142116006
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Official Citation:Kevin J. Luebke and Peter B. Dervan Nonenzymatic ligation of double-helical DNA by alternate-strand triple helix formation Nucl. Acids Res. (1992) 20 (12): 3005-3009 doi:10.1093/nar/20.12.3005
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66943
Deposited By: Victoria Brennan
Deposited On:19 May 2016 17:30
Last Modified:11 Nov 2021 00:24

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