The influence of single base triplet changes on the stability of a Pur·Pur·Pyr triple helix determined by affinity cleaving

Beal, Peter A. and Dervan, Peter B. (1992) The influence of single base triplet changes on the stability of a Pur·Pur·Pyr triple helix determined by affinity cleaving. Nucleic Acids Research, 20 (11). pp. 2773-2776. ISSN 0305-1048. PMCID PMC336921. doi:10.1093/nar/20.11.2773. https://resolver.caltech.edu/CaltechAUTHORS:20160510-142116893

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Abstract

The influence of sixteen base triplet changes at a single position within a pur·pur·pyr triple helix was examined by affinity cleaving. For the 15 base pair target site studied here, G·GC, A·AT and T·AT triplets stabilize a triple helix to a greater extent than the other 13 natural triplets (pH = 7.4, 25°C). Weaker interactions were detected for the C·AT, A·GC and T·CG triplets. The absence of specific, highly stabilizing interactions between third strand bases and the CG or TA base pairs demonstrates a current sequence limitation to formation of this structure. Models for the two dimensional base triplet interactions for all possible 16 natural triplets are presented.

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1093/nar/20.11.2773	DOI	Article
http://nar.oxfordjournals.org/content/20/11/2773	Publisher	Article
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC336921/	PubMed Central	Article

ORCID:

Author	ORCID
Dervan, Peter B.	0000-0001-8852-7306

Additional Information:

© 1992 Oxford University Press. Received February 20, 1992; Revised and Accepted May 5, 1992. We are grateful for generous support from the National Institutes of Health (HG00329) and a DOE predoctoral fellowship for P.A.B.

Funders:

Funding Agency	Grant Number
NIH	HG00329
Department of Energy (DOE)	UNSPECIFIED

Issue or Number:

PubMed Central ID:

PMC336921

DOI:

10.1093/nar/20.11.2773

Record Number:

CaltechAUTHORS:20160510-142116893

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20160510-142116893

Official Citation:

Peter A. Beal and Peter B. Dervan The influence of single base triplet changes on the stability of a Pur·Pur·Pyr triple helix determined by affinity cleaving Nucl. Acids Res. (1992) 20 (11): 2773-2776 doi:10.1093/nar/20.11.2773

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ID Code:

66944

Collection:

CaltechAUTHORS

Deposited By:

Victoria Brennan

Deposited On:

19 May 2016 17:28

Last Modified:

11 Nov 2021 00:24

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