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The influence of single base triplet changes on the stability of a Pur·Pur·Pyr triple helix determined by affinity cleaving

Beal, Peter A. and Dervan, Peter B. (1992) The influence of single base triplet changes on the stability of a Pur·Pur·Pyr triple helix determined by affinity cleaving. Nucleic Acids Research, 20 (11). pp. 2773-2776. ISSN 0305-1048. PMCID PMC336921. doi:10.1093/nar/20.11.2773. https://resolver.caltech.edu/CaltechAUTHORS:20160510-142116893

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Abstract

The influence of sixteen base triplet changes at a single position within a pur·pur·pyr triple helix was examined by affinity cleaving. For the 15 base pair target site studied here, G·GC, A·AT and T·AT triplets stabilize a triple helix to a greater extent than the other 13 natural triplets (pH = 7.4, 25°C). Weaker interactions were detected for the C·AT, A·GC and T·CG triplets. The absence of specific, highly stabilizing interactions between third strand bases and the CG or TA base pairs demonstrates a current sequence limitation to formation of this structure. Models for the two dimensional base triplet interactions for all possible 16 natural triplets are presented.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1093/nar/20.11.2773DOIArticle
http://nar.oxfordjournals.org/content/20/11/2773PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC336921/PubMed CentralArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1992 Oxford University Press. Received February 20, 1992; Revised and Accepted May 5, 1992. We are grateful for generous support from the National Institutes of Health (HG00329) and a DOE predoctoral fellowship for P.A.B.
Funders:
Funding AgencyGrant Number
NIHHG00329
Department of Energy (DOE)UNSPECIFIED
Issue or Number:11
PubMed Central ID:PMC336921
DOI:10.1093/nar/20.11.2773
Record Number:CaltechAUTHORS:20160510-142116893
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-142116893
Official Citation:Peter A. Beal and Peter B. Dervan The influence of single base triplet changes on the stability of a Pur·Pur·Pyr triple helix determined by affinity cleaving Nucl. Acids Res. (1992) 20 (11): 2773-2776 doi:10.1093/nar/20.11.2773
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66944
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:19 May 2016 17:28
Last Modified:11 Nov 2021 00:24

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