Beal, Peter A. and Dervan, Peter B. (1992) The influence of single base triplet changes on the stability of a Pur·Pur·Pyr triple helix determined by affinity cleaving. Nucleic Acids Research, 20 (11). pp. 2773-2776. ISSN 0305-1048. PMCID PMC336921. doi:10.1093/nar/20.11.2773. https://resolver.caltech.edu/CaltechAUTHORS:20160510-142116893
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Abstract
The influence of sixteen base triplet changes at a single position within a pur·pur·pyr triple helix was examined by affinity cleaving. For the 15 base pair target site studied here, G·GC, A·AT and T·AT triplets stabilize a triple helix to a greater extent than the other 13 natural triplets (pH = 7.4, 25°C). Weaker interactions were detected for the C·AT, A·GC and T·CG triplets. The absence of specific, highly stabilizing interactions between third strand bases and the CG or TA base pairs demonstrates a current sequence limitation to formation of this structure. Models for the two dimensional base triplet interactions for all possible 16 natural triplets are presented.
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Additional Information: | © 1992 Oxford University Press. Received February 20, 1992; Revised and Accepted May 5, 1992. We are grateful for generous support from the National Institutes of Health (HG00329) and a DOE predoctoral fellowship for P.A.B. | ||||||||||||
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Issue or Number: | 11 | ||||||||||||
PubMed Central ID: | PMC336921 | ||||||||||||
DOI: | 10.1093/nar/20.11.2773 | ||||||||||||
Record Number: | CaltechAUTHORS:20160510-142116893 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-142116893 | ||||||||||||
Official Citation: | Peter A. Beal and Peter B. Dervan The influence of single base triplet changes on the stability of a Pur·Pur·Pyr triple helix determined by affinity cleaving Nucl. Acids Res. (1992) 20 (11): 2773-2776 doi:10.1093/nar/20.11.2773 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 66944 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | Victoria Brennan | ||||||||||||
Deposited On: | 19 May 2016 17:28 | ||||||||||||
Last Modified: | 11 Nov 2021 00:24 |
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