Kiessling, Laura L. and Griffin, Linda C. and Dervan, Peter B. (1992) Flanking sequence effects within the pyrimidine triple-helix motif characterized by affinity cleaving. Biochemistry, 31 (10). pp. 2829-2834. ISSN 0006-2960. doi:10.1021/bi00125a026. https://resolver.caltech.edu/CaltechAUTHORS:20160510-142118738
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Abstract
Nearest neighbor interactions affect the stabilities of triple-helical complexes. Within a pyrimidine triple-helical motif, the relative stabilities of natural base triplets T·AT, C+GC, and G·TA, as well as triplets, D_3·TA and D_3·CG, containing the nonnatural deoxyribonucleoside 1-(2-deoxy-β-D-ribofuranosyl)-4-(3- benzamido)phenylimidazole (D_3) were characterized by the affinity cleaving method in the context of different flanking triplets (T·AT, T·AT; T·AT, C+GC; C+GC, T·AT; G+GC, C+GC). The T·AT triplet was shown to be insensitive to substitutions in either the 3' or 5' directions, while the relative stabilities of triple helices containing C+GC triplets decreased as the number of adjacent C+GC triplets increased. Triple helices incorporating a G·TA interaction were most stable when this triplet was flanked by two T·AT triplets and were adversely affected when a C+GC triplet was placed in the adjacent 5' direction. Similarly, complexes containing D_3·TA or D_3·CG triplets were most stable when the triplet was flanked by two T·AT triplets but were destabilized when the adjacent 3' neighbor position was occupied with a C+GC triplet. This information regarding sequence composition effects in triple-helix formation establishes a set of guidelines for targeting sequences of double-helical DNA by the pyrimidine triple-helix motif.
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| Additional Information: | © 1992 American Chemical Society. Received September 3, 1991; Revised Manuscript Received December 30, 1991. We are grateful to the Office of Naval Research for generous support. We are grateful to Scott Singleton for helpful discussions and to the American Cancer Society for a postdoctoral fellowship to L.L.K. | |||||||||
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| Issue or Number: | 10 | |||||||||
| DOI: | 10.1021/bi00125a026 | |||||||||
| Record Number: | CaltechAUTHORS:20160510-142118738 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-142118738 | |||||||||
| Official Citation: | Flanking sequence effects within the pyrimidine triple-helix motif characterized by affinity cleaving Laura L. Kiessling, Linda C. Griffin, and Peter B. Dervan Biochemistry 1992 31 (10), 2829-2834 DOI: 10.1021/bi00125a026 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 66945 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Victoria Brennan | |||||||||
| Deposited On: | 19 May 2016 17:33 | |||||||||
| Last Modified: | 11 Nov 2021 00:24 |
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