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Cooperative binding of oligonucleotides to DNA by triple helix formation: dimerization via Watson-Crick hydrogen bonds

Distefano, Mark D. and Shin, Jumi A. and Dervan, Peter B. (1991) Cooperative binding of oligonucleotides to DNA by triple helix formation: dimerization via Watson-Crick hydrogen bonds. Journal of the American Chemical Society, 113 (15). pp. 5901-5902. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20160510-145312296

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Abstract

[Introduction] Cooperative binding by proteins to DNA results in higher sequence specificity as well as greater sensitivity to concentration changes. We recently reported cooperative binding of two oligonucleotides at abutting sites by triple helix formation on double helical DNA. However, the enhanced binding observed was modest (a factor of 3.5) and likely due to favorable basestacking interactions between adjacent oligonucleotides and/or induced conformational changes propagated to adjacent binding sites. Thus, the issue arises whether cooperativity in oligonucleotide-directed triple helix formation can be enhanced by the addition of discrete dimerization domains. We report here the binding properties of oligonucleotides that dimerize by Watson-Crick hydrogen bonds and bind neighboring sites on double helical DNA by triple helix formation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja00015a076DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00015a076PublisherArticle
Additional Information:© 1991 American Chemical Society. Received March 20, 1991. This work was supported by the National Institutes of Health (GM 35724), Fellowship DRG-1003 of the Damon Runyon-Walter Winchell Cancer Research Fund to M.D.D., and an N.I.H. predoctoral fellowship for J.A.S.
Funders:
Funding AgencyGrant Number
NIHGM-35724
Damon Runyon-Walter Winchell Cancer ResearchDRG-1003
NIH Predoctoral FellowshipUNSPECIFIED
Issue or Number:15
Record Number:CaltechAUTHORS:20160510-145312296
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-145312296
Official Citation:Cooperative binding of oligonucleotides to DNA by triple helix formation: dimerization via Watson-Crick hydrogen bonds Mark D. Distefano, Jumi A. Shin, and Peter B. Dervan Journal of the American Chemical Society 1991 113 (15), 5901-5902 DOI: 10.1021/ja00015a076
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66949
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:19 May 2016 18:20
Last Modified:03 Oct 2019 10:01

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