Povsic, Thomas J. and Dervan, Peter B. (1990) Sequence-specific alkylation of double-helical DNA by oligonucleotide-directed triple-helix formation. Journal of the American Chemical Society, 112 (25). pp. 9428-9430. ISSN 0002-7863. doi:10.1021/ja00181a075. https://resolver.caltech.edu/CaltechAUTHORS:20160510-145543263
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Abstract
Affinity cleaving, a method that relies on the attachment of a nonspecific cleaving moiety, such as EDTA•Fe(ll), to a DNA binding molecule, facilitates the elucidation of the structural principles for DNA recognition. The determination of the sequence specificities, groove locations, and binding orientations of peptide analogues, protein-DNA binding motifs, and oligonucleotide-triple-helix motifs has provided reliable models for the sequence-specific recognition of double-helical DNA. It now becomes possible to combine these binding molecules with domains capable of base-specific and quantitative modification of DNA (Figure 1). We report the design and synthesis of an ligodeoxyribonucleotide equipped with an electrophile at the 5'-end that binds to double-helical DNA by triple-helix formation and alkylates predominantly at a single guanine base adjacent to the target DNA sequence in high yield.
Item Type: | Article | |||||||||
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Additional Information: | © 1990 American Chemical Society. Received July 2, 1990. We are grateful for generous support from the National Institutes of Health (GM-35724) and a Rainin predoctoral fellowship to T.J.P. | |||||||||
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Issue or Number: | 25 | |||||||||
DOI: | 10.1021/ja00181a075 | |||||||||
Record Number: | CaltechAUTHORS:20160510-145543263 | |||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-145543263 | |||||||||
Official Citation: | Sequence-specific alkylation of double-helical DNA by oligonucleotide-directed triple-helix formation Thomas J. Povsic and Peter B. Dervan Journal of the American Chemical Society 1990 112 (25), 9428-9430 DOI: 10.1021/ja00181a075 | |||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
ID Code: | 66950 | |||||||||
Collection: | CaltechAUTHORS | |||||||||
Deposited By: | Victoria Brennan | |||||||||
Deposited On: | 19 May 2016 18:24 | |||||||||
Last Modified: | 11 Nov 2021 00:24 |
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