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Sequence-specific alkylation of double-helical DNA by oligonucleotide-directed triple-helix formation

Povsic, Thomas J. and Dervan, Peter B. (1990) Sequence-specific alkylation of double-helical DNA by oligonucleotide-directed triple-helix formation. Journal of the American Chemical Society, 112 (25). pp. 9428-9430. ISSN 0002-7863. doi:10.1021/ja00181a075.

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Affinity cleaving, a method that relies on the attachment of a nonspecific cleaving moiety, such as EDTA•Fe(ll), to a DNA binding molecule, facilitates the elucidation of the structural principles for DNA recognition. The determination of the sequence specificities, groove locations, and binding orientations of peptide analogues, protein-DNA binding motifs, and oligonucleotide-triple-helix motifs has provided reliable models for the sequence-specific recognition of double-helical DNA. It now becomes possible to combine these binding molecules with domains capable of base-specific and quantitative modification of DNA (Figure 1). We report the design and synthesis of an ligodeoxyribonucleotide equipped with an electrophile at the 5'-end that binds to double-helical DNA by triple-helix formation and alkylates predominantly at a single guanine base adjacent to the target DNA sequence in high yield.

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Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1990 American Chemical Society. Received July 2, 1990. We are grateful for generous support from the National Institutes of Health (GM-35724) and a Rainin predoctoral fellowship to T.J.P.
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Rainin Predoctoral FellowshipUNSPECIFIED
Issue or Number:25
Record Number:CaltechAUTHORS:20160510-145543263
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Official Citation:Sequence-specific alkylation of double-helical DNA by oligonucleotide-directed triple-helix formation Thomas J. Povsic and Peter B. Dervan Journal of the American Chemical Society 1990 112 (25), 9428-9430 DOI: 10.1021/ja00181a075
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66950
Deposited By: Victoria Brennan
Deposited On:19 May 2016 18:24
Last Modified:11 Nov 2021 00:24

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