Strobel, S. A. and Dervan, P. B. (1992) Triple helix-mediated single-site enzymatic cleavage of megabase genomic DNA. In: Recombinant DNA. Methods in Enzymology. Vol.Part G. No.216. Elsevier , New York, NY, pp. 309-321. ISBN 978-0-12-182117-3. https://resolver.caltech.edu/CaltechAUTHORS:20160510-145809040
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Abstract
This chapter explains the triple helix-mediated single-site enzymatic cleavage of megabase genomic DNA. Oligonucleotide-directed triple helix formation is a generalizable chemical approach for the recognition and cleavage of a single target site within several megabase pairs of duplex genomic DNA. The generalizability of triple helix-mediated enzymatic cleavage affords high specificity that can be readily customized to unique genetic markers without artificial insertion of a target sequence. Extensive sequencing to identify target sites could be avoided by using degenerate oligonucleotides to screen genetic markers for overlapping triple helix/ endonuclease sites. The potential generalizability of triple helix-mediated Achilles' heel cleavage, a technique capable of near-quantitative cleavage at a single site in at least 14 megabase pairs of DNA, could assist in physical mapping of chromosomal DNA and expedite isolation of DNA segments linked to the disease.
| Item Type: | Book Section | |||||||||
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| Additional Information: | © 1992 Elsevier Inc. We are grateful to Dr. John Hanish for helpful discussions, the National Institutes of Health for grant support, and the Howard Hughes Medical Institute for a predoctoral fellowship to S.A.S. | |||||||||
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| Series Name: | Methods in Enzymology | |||||||||
| Issue or Number: | 216 | |||||||||
| DOI: | 10.1016/0076-6879(92)16029-J | |||||||||
| Record Number: | CaltechAUTHORS:20160510-145809040 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-145809040 | |||||||||
| Official Citation: | Scott A. Strobel, Peter B. Dervan, [27] Triple helix-mediated single-site enzymatic cleavage of megabase genomic DNA, Methods in Enzymology, Academic Press, 1992, Volume 216, Pages 309-321, ISSN 0076-6879, ISBN 9780121821173, http://dx.doi.org/10.1016/0076-6879(92)16029-J. (http://www.sciencedirect.com/science/article/pii/007668799216029J) | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 66952 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Victoria Brennan | |||||||||
| Deposited On: | 19 May 2016 18:19 | |||||||||
| Last Modified: | 11 Nov 2021 00:24 |
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