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Triple helix-mediated single-site enzymatic cleavage of megabase genomic DNA

Strobel, S. A. and Dervan, P. B. (1992) Triple helix-mediated single-site enzymatic cleavage of megabase genomic DNA. In: Recombinant DNA. Methods in Enzymology. Vol.Part G. No.216. Elsevier , New York, NY, pp. 309-321. ISBN 978-0-12-182117-3.

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This chapter explains the triple helix-mediated single-site enzymatic cleavage of megabase genomic DNA. Oligonucleotide-directed triple helix formation is a generalizable chemical approach for the recognition and cleavage of a single target site within several megabase pairs of duplex genomic DNA. The generalizability of triple helix-mediated enzymatic cleavage affords high specificity that can be readily customized to unique genetic markers without artificial insertion of a target sequence. Extensive sequencing to identify target sites could be avoided by using degenerate oligonucleotides to screen genetic markers for overlapping triple helix/ endonuclease sites. The potential generalizability of triple helix-mediated Achilles' heel cleavage, a technique capable of near-quantitative cleavage at a single site in at least 14 megabase pairs of DNA, could assist in physical mapping of chromosomal DNA and expedite isolation of DNA segments linked to the disease.

Item Type:Book Section
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URLURL TypeDescription Chapter Chapter
Dervan, P. B.0000-0001-8852-7306
Additional Information:© 1992 Elsevier Inc. We are grateful to Dr. John Hanish for helpful discussions, the National Institutes of Health for grant support, and the Howard Hughes Medical Institute for a predoctoral fellowship to S.A.S.
Funding AgencyGrant Number
Howard Hughes Medical Institute (HHMI)UNSPECIFIED
Series Name:Methods in Enzymology
Issue or Number:216
Record Number:CaltechAUTHORS:20160510-145809040
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Official Citation:Scott A. Strobel, Peter B. Dervan, [27] Triple helix-mediated single-site enzymatic cleavage of megabase genomic DNA, Methods in Enzymology, Academic Press, 1992, Volume 216, Pages 309-321, ISSN 0076-6879, ISBN 9780121821173, (
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66952
Deposited By: Victoria Brennan
Deposited On:19 May 2016 18:19
Last Modified:11 Nov 2021 00:24

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