Effects of an abasic site on triple helix formation characterized by affinity cleaving

Horne, David A. and Dervan, Peter B. (1991) Effects of an abasic site on triple helix formation characterized by affinity cleaving. Nucleic Acids Research, 19 (18). pp. 4963-4965. ISSN 0305-1048. PMCID PMC328797. doi:10.1093/nar/19.18.4963. https://resolver.caltech.edu/CaltechAUTHORS:20160510-145811847

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Abstract

The stability of triple helical complexes of pyrimidine oligodeoxyribonucleotides containing one abasic 1,2- dideoxy-D-ribose (ø) residue was examined by affinity cleaving. Within a pyrimidine third strand, the triplets ø·AT, ø·GC, ø·TA and ø·CG are significantly less stable than the triplets, T- AT, C + GC and GTA. The decrease in binding produced by an abasic residue is similar to that observed with imperfectly matched natural base triplets, with ø · AT and ø · GC being less stable than ø · TA and ø · CG triplets for the sequences studied.

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1093/nar/19.18.4963	DOI	Article
http://nar.oxfordjournals.org/content/19/18/4963	Publisher	Article
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC328797/	PubMed Central	Article

ORCID:

Author	ORCID
Dervan, Peter B.	0000-0001-8852-7306

Additional Information:

© 1991 Oxford University Press. Received June 7, 1991; Revised and Accepted August 9, 1991. We are grateful for grant support from the National Institutes of Health, the Office of Naval Research, an unrestricted research grant from Burroughs-Wellcome, and a National Institutes of Health postdoctoral fellowship from the National Institutes of General Medical Science (D.A.H.).

Funders:

Funding Agency	Grant Number
Office of Naval Research (ONR)	UNSPECIFIED
Burroughs-Wellcome	UNSPECIFIED
NIH Postdoctoral Fellowship	UNSPECIFIED

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PubMed Central ID:

PMC328797

DOI:

10.1093/nar/19.18.4963

Record Number:

CaltechAUTHORS:20160510-145811847

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20160510-145811847

Official Citation:

David A. Horne and Peter B. Dervan Effects of an abasic site on triple helix formation characterized by affinity cleaving Nucl. Acids Res. (1991) 19 (18): 4963-4965 doi:10.1093/nar/19.18.4963

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ID Code:

66954

Collection:

CaltechAUTHORS

Deposited By:

Victoria Brennan

Deposited On:

19 May 2016 18:35

Last Modified:

11 Nov 2021 00:24

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