Häner, Robert and Dervan, Peter B. (1990) Single-strand DNA triple-helix formation. Biochemistry, 29 (42). pp. 9761-9765. ISSN 0006-2960. https://resolver.caltech.edu/CaltechAUTHORS:20160510-145813593
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Abstract
Chemical modification studies provide evidence that single-stranded oligodeoxyribonucleotides can form stable intrastrand triple helices. Two oligonucleotides of opposite polarity were synthesized, each composed of a homopurine-homopyrimidine hairpin stem linked to a pyrimidine sequence which is capable of folding back on the hairpin stem and forming specific Hoogsteen hydrogen bonds. Using potassium permanganate as a chemical modification reagent, we have found that two oligodeoxyribonucleotides of sequence composition type 5'-(purine)_8(N)_4(pyrimidine)_8(N)_6(pyrimidine)_8-3' and 5'-(pyrimidine)_8N_6(pyrimidine)_8N_4(purine)_8-3' undergo dramatic structural changes consistent with intrastrand DNA triple-helix formation induced by lowering the pH or raising the Mg^(2+) concentration. The intrastrand DNA triple helix is sensitive to base mismatches.
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| Additional Information: | © 1990 ACS. Received July 13, 1990; Revised Manuscript Received August 17, 1990. Supported by NIH Grant GM35724. We are grateful to the National Institutes of Health for generous support and to Sandoz, Basel, for a postdoctoral fellowship to R.H. | |||||||||
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| Issue or Number: | 42 | |||||||||
| Record Number: | CaltechAUTHORS:20160510-145813593 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20160510-145813593 | |||||||||
| Official Citation: | Single-strand DNA triple-helix formation Robert Haner and Peter B. Dervan Biochemistry 1990 29 (42), 9761-9765 DOI: 10.1021/bi00494a001 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 66956 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Victoria Brennan | |||||||||
| Deposited On: | 19 May 2016 21:41 | |||||||||
| Last Modified: | 26 Nov 2019 11:15 |
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