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Single-strand DNA triple-helix formation

Häner, Robert and Dervan, Peter B. (1990) Single-strand DNA triple-helix formation. Biochemistry, 29 (42). pp. 9761-9765. ISSN 0006-2960. doi:10.1021/bi00494a001.

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Chemical modification studies provide evidence that single-stranded oligodeoxyribonucleotides can form stable intrastrand triple helices. Two oligonucleotides of opposite polarity were synthesized, each composed of a homopurine-homopyrimidine hairpin stem linked to a pyrimidine sequence which is capable of folding back on the hairpin stem and forming specific Hoogsteen hydrogen bonds. Using potassium permanganate as a chemical modification reagent, we have found that two oligodeoxyribonucleotides of sequence composition type 5'-(purine)_8(N)_4(pyrimidine)_8(N)_6(pyrimidine)_8-3' and 5'-(pyrimidine)_8N_6(pyrimidine)_8N_4(purine)_8-3' undergo dramatic structural changes consistent with intrastrand DNA triple-helix formation induced by lowering the pH or raising the Mg^(2+) concentration. The intrastrand DNA triple helix is sensitive to base mismatches.

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Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1990 ACS. Received July 13, 1990; Revised Manuscript Received August 17, 1990. Supported by NIH Grant GM35724. We are grateful to the National Institutes of Health for generous support and to Sandoz, Basel, for a postdoctoral fellowship to R.H.
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Issue or Number:42
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Official Citation:Single-strand DNA triple-helix formation Robert Haner and Peter B. Dervan Biochemistry 1990 29 (42), 9761-9765 DOI: 10.1021/bi00494a001
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66956
Deposited By: Victoria Brennan
Deposited On:19 May 2016 21:41
Last Modified:11 Nov 2021 00:24

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