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Recognition of mixed-sequence duplex DNA by alternate-strand triple-helix formation

Horne, David A. and Dervan, Peter B. (1990) Recognition of mixed-sequence duplex DNA by alternate-strand triple-helix formation. Journal of the American Chemical Society, 112 (6). pp. 2435-2437. ISSN 0002-7863. https://resolver.caltech.edu/CaltechAUTHORS:20160510-151908781

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Abstract

Oligodeoxyribonucleotide-directed triple-helix formation offers a chemical approach for the sequence-specific binding of double-helical DNA that is 10^6 times more specific than restriction enzymes. Because triple-helix formation by pyrimidine oligonucleotides is limited to purine tracts, it is desirable to find a general solution whereby oligonucleotides could be used to bind all four base pairs of intact duplex DNA (37 °C, pH 7.0). Approaches toward such a goal include the following: the search for other natural triplet specificities, such as G-TA triplets; the design of nonnatural bases for completion of the triplet code; the incorporation of abasic residues for nonreading of certain base pairs; and the design of oligonucleotides capable of binding alternate strands of duplex DNA by triple-helix formation. We report that a pyrimidine oligodeoxyribonucleotide-EDTA-Fe containing a 3'-3' phosphodiester and a 1,2-dideoxy-~ribose linker binds and cleaves a mixed-sequence double-helical DNA target site by alternate-strand triple-helix formation.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ja00162a063DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00162a063PublisherArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1990 American Chemical Society. Received December 8, 1989. We are grateful for grant support from the National Institutes of Health (GM-42966), an unrestricted research grant from Burroughs-Wellcome, and a National Institutes of Health postdoctoral fellowship from the National Institute of General Medical Science (D.A.H.)
Funders:
Funding AgencyGrant Number
NIHGM-42966
Burroughs-WellcomeUNSPECIFIED
NIH Postdoctoral FellowshipUNSPECIFIED
Issue or Number:6
Record Number:CaltechAUTHORS:20160510-151908781
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-151908781
Official Citation:Recognition of mixed-sequence duplex DNA by alternate-strand triple-helix formation David A. Horne and Peter B. Dervan Journal of the American Chemical Society 1990 112 (6), 2435-2437 DOI: 10.1021/ja00162a063
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66960
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:18 May 2016 23:40
Last Modified:26 Nov 2019 11:15

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