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Nonenzymatic ligation of oligodeoxyribonucleotides on a duplex DNA template by triple-helix formation

Luebke, Kevin J. and Dervan, Peter B. (1989) Nonenzymatic ligation of oligodeoxyribonucleotides on a duplex DNA template by triple-helix formation. Journal of the American Chemical Society, 111 (23). pp. 8733-8735. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20160510-152740060

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Abstract

We report that a double-stranded DNA template can direct the sequence-specific formation of a phosphodiester linkage between oligodeoxyribonucleotides in aqueous solution by juxtaposing the termini head-to-tail in a triple-helical complex (Figure 1). A triple-stranded complex can be formed when a pyrimidine oligodeoxynucleotide binds in the major groove of duplex DNA parallel to the purine Watson-Crick strand. In the complex, sequence specificity for the condensation of oligodeoxynucleotides is directed by Hoogsteen base pairing.


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http://dx.doi.org/10.1021/ja00205a033DOIArticle
http://pubs.acs.org/doi/abs/10.1021/ja00205a033PublisherArticle
Additional Information:© 1989 American Chemical Society. Received August 2, 1989. This work was supported in part by the National Institutes of Health (GM-35724) and the Caltech Consortium in Chemistry and Chemical Engineering (founding members: E. I. du Pont de Nemours and Company, Inc., Eastman Kodak Company, Minnesota Mining and Manufacturing Company, and Shell Oil Company Foundation). We are grateful for a National Research Service Award to K.J.L. from the National Institute of General Medical Science.
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Funding AgencyGrant Number
NIHGM-35724
Caltech Consortium in Chemistry and Chemical EngineeringUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Record Number:CaltechAUTHORS:20160510-152740060
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20160510-152740060
Official Citation:Nonenzymatic ligation of oligodeoxyribonucleotides on a duplex DNA template by triple-helix formation Kevin J. Luebke and Peter B. Dervan Journal of the American Chemical Society 1989 111 (23), 8733-8735 DOI: 10.1021/ja00205a033
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ID Code:66961
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:19 May 2016 18:46
Last Modified:19 May 2016 18:46

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