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Orientation of the putative recognition helix in the DNA-binding domain of Hin recombinase complexed with the hix site

Mack, David P. and Sluka, James P. and Shin, Jumi A. and Griffin, John H. and Simon, Melvin I. and Dervan, Peter B. (1990) Orientation of the putative recognition helix in the DNA-binding domain of Hin recombinase complexed with the hix site. Biochemistry, 29 (28). pp. 6561-6567. ISSN 0006-2960. https://resolver.caltech.edu/CaltechAUTHORS:20160510-155632013

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Abstract

On the basis of sequence similarity with other known DNA-binding proteins, the DNA-binding domain of Hin recombinase, residues 139-190, is thought to bind DNA by a helix-turn-helix motif. Two models can be considered that differ in the orientation of the recognition helix in the major groove of DNA. One is based on the orientation of the recognition helix found in the 434 repressor (1-69) and lambda repressor-DNA cocrystals, and the other is based on the NMR studies of lac repressor headpiece. Cleavage by EDTA.Fe attached to a lysine side chain (Ser^(183) → Lys^(183)) near the COOH terminus of Hin(139-184) reveals that the putative recognition helix is oriented toward the center of the inverted repeats in a manner similar to that seen in the 434 and λ repressor-DNA cocrystals.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/bi00480a003DOIArticle
http://pubs.acs.org/doi/abs/10.1021/bi00480a003PublisherArticle
Additional Information:© 1990 American Chemical Society. Received January 5, 1990; Revised Manuscript Received March 6, 1990. Supported by grants from the DARPA University Research Initiation Program, the National Foundation for Cancer Research, and the NSF and by National Research Service Awards from NIGMS to D.P.M., J.P.S., and J.A.S.
Funders:
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
National Foundation for Cancer ResearchUNSPECIFIED
NSFUNSPECIFIED
NIH Predoctoral FellowshipUNSPECIFIED
Issue or Number:28
Record Number:CaltechAUTHORS:20160510-155632013
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-155632013
Official Citation:Orientation of the putative recognition helix in the DNA-binding domain of Hin recombinase complexed with the Hix site David P. Mack, James P. Sluka, Jumi A. Shin, John H. Griffin, Melvin I. Simon, and Peter B. Dervan Biochemistry 1990 29 (28), 6561-6567 DOI: 10.1021/bi00480a003
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66964
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:18 May 2016 23:54
Last Modified:03 Oct 2019 10:01

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