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Structural motif of the GCN4 DNA binding domain characterized by affinity cleaving

Oakley, Martha G. and Dervan, Peter B. (1990) Structural motif of the GCN4 DNA binding domain characterized by affinity cleaving. Science, 248 (4957). pp. 847-850. ISSN 0036-8075. https://resolver.caltech.edu/CaltechAUTHORS:20160510-155633674

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Abstract

The NH_2-terminal locations of a dimer containing the DNA binding domain of the yeast transcriptional activator GCN4 have been mapped on the binding sites 5'-CTGACTAAT-3' and 5'-ATGACTCTT-3'. Affinity cleaving was effected by synthetic GCN4 proteins with Fe.EDTA moieties at the NH2-terminus. Analysis of the DNA cleavage patterns for dimers of the Fe·EDTA-proteins corresponding to GCN4 residues 222 to 281 and 226 to 281 revealed that the NH2-termini were in the major groove nine to ten base pairs apart and were symmetrically displaced four to five base pairs from the central C of the recognition site. This result is consistent with the Y-shaped scissor grip-leucine zipper model recently proposed for a class of DNA binding proteins important in the regulation of gene expression.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1126/science.2111578DOIArticle
http://science.sciencemag.org/content/248/4957/847PublisherArticle
ORCID:
AuthorORCID
Dervan, Peter B.0000-0001-8852-7306
Additional Information:© 1990 American Association for the Advancement of Science. 16 January 1990; accepted 23 March 1990. Supported by grants from the DARPA University Research Initiative Program, the National Foundation for Cancer Research, and an NSF predoctoral fellowship to M.G.O. We thank C. S. Parker for the plasmid pARE/GCRE (18).
Funders:
Funding AgencyGrant Number
Defense Advanced Research Projects Agency (DARPA)UNSPECIFIED
National Foundation for Cancer ResearchUNSPECIFIED
NSF Predoctoral FellowshipUNSPECIFIED
Issue or Number:4957
Record Number:CaltechAUTHORS:20160510-155633674
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20160510-155633674
Official Citation:Structural motif of the GCN4 DNA binding domain characterized by affinity cleaving MG Oakley, PB Dervan Science 18 May 1990: Vol. 248, Issue 4957, pp. 847-850 DOI: 10.1126/science.2111578
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:66966
Collection:CaltechAUTHORS
Deposited By: Victoria Brennan
Deposited On:19 May 2016 21:17
Last Modified:26 Nov 2019 11:15

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