Strobel, Scott A. and Moser, Heinz E. and Dervan, Peter B. (1988) Double strand cleavage of genomic DNA at a single site by triple helix formation. Journal of the American Chemical Society, 110 (23). pp. 7927-7929. ISSN 0002-7863. http://resolver.caltech.edu/CaltechAUTHORS:20160511-135304681
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Abstract
The sequence-specific cleavage of double helical DNA by restriction endonucleases is essential for many techniques in molecular biology, including gene isolation, DNA sequencing, and recombinant DNA manipulations. With the advent of pulsed-field gel electrophoresis, the separation of large segments of DNA is now possible. However, the recognition sequences of naturally occurring restriction enzymes are in the range of 4-8 base pairs, and hence their sequence specificites may be inadequate for isolating genes from large chromosomes (10^8 base pairs in size) or mapping genomic DNA.
| Item Type: | Article | |||||||||
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| Additional Information: | © 1988 American Chemical Society. Received July 22, 1988. Publication Date: November 1988. This work was supported in part by the National Institutes of Health (GM 35724), the Caltech Consortium in Chemistry and Chemical Engineering (Founding members: E. I. duPont de Nemours and Company, Inc., Eastman Kodak Company, Minnesota Mining and Manufacturing Company, and Shell Oil Company Foundation), and a National Research Service Award (T32GM07616) from the NIH-GM to S.A.S. | |||||||||
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| Record Number: | CaltechAUTHORS:20160511-135304681 | |||||||||
| Persistent URL: | http://resolver.caltech.edu/CaltechAUTHORS:20160511-135304681 | |||||||||
| Official Citation: | Double strand cleavage of genomic DNA at a single site by triple helix formation Scott A. Strobel, Heinz E. Moser, and Peter B. Dervan Journal of the American Chemical Society 1988 110 (23), 7927-7929 DOI: 10.1021/ja00231a082 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 67001 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Victoria Brennan | |||||||||
| Deposited On: | 17 May 2016 16:52 | |||||||||
| Last Modified: | 17 May 2016 23:32 |
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